Interactions between sub-10 nm iron and cerium oxide nanoparticles and 3T3 fibroblasts : the role of the coating and aggregation state - Condensed Matter > Materials ScienceReportar como inadecuado




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Abstract: Recent nanotoxicity studies revealed that the physico-chemicalcharacteristics of engineered nanomaterials play an important role in theinteractions with living cells. Here, we report on the toxicity and uptake ofthe cerium and iron oxide sub-10 nm nanoparticles by NIH-3T3 mouse fibroblasts.Coating strategies include low-molecular weight ligands citric acid andpolymers polyacrylic acid, MW = 2000 g mol-1. Electrostatically adsorbed onthe surfaces, the organic moieties provide a negatively charged coating inphysiological conditions. We find that most particles were biocompatible, asexposed cells remained 100% viable relative to controls. Only the bare and thecitrate-coated nanoceria exhibit a slight decrease of the mitochondrialactivity for cerium concentrations above 5 mM equivalent to 0.8 g L-1. Wealso observe that the citrate-coated particles are internalized by the cells inlarge amounts, typically 250 pg per cell after a 24 h incubation for ironoxide. In contrast, the polymer-coated particles are taken up at much lowerrates < 30 pg per cell. The strong uptake shown by the citrate-coatedparticles is related to the destabilization of the dispersions in the cellculture medium and their sedimentation down to the cell membranes. Inconclusion, we show that the uptake of nanomaterials by living cells depends onthe coating of the particles and on its ability to preserve the colloidalnature of the dispersions.



Autor: M. Safi, H. Sarrouj, O. Sandre, N. Mignet, J.-F. Berret

Fuente: https://arxiv.org/



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