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Müller glia provide tensile strength to the developing retina

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Publication Date: 2015-09-28

Journal Title: Journal of Cell Biology

Publisher: Rockefeller University Press

Volume: 210

Issue: 7

Pages: 1075-1083

Language: English

Type: Article

Metadata: Show full item record

Citation: MacDonald, R. B., Randlett, O., Oswald, J., Yoshimatsu, T., Franze, K., & Harris, W. A. (2015). Müller glia provide tensile strength to the developing retina. Journal of Cell Biology, 210 (7), 1075-1083.

Description: This is the final version of the article. It first appeared from the Rockefeller Press via http://dx.doi.org/10.1083/jcb.201503115

Abstract: To investigate the cellular basis of tissue integrity in a vertebrate CNS tissue, we eliminated Müller glial cells (MG) from the zebrafish retina. For well over a century glial cells have been ascribed a mechanical role in the support of neural tissues, yet this idea has not been specifically tested in vivo. We report here that retinas devoid of MG rip apart – a defect known as retinoschisis. Using atomic force microscopy, we show that retinas without MG have decreased resistance to tensile stress and are softer than controls. Laser ablation of MG processes showed that these cells are under tension in the tissue. Thus, we propose that MG act like springs that hold the neural retina together, finally confirming an active mechanical role of glial cells in the CNS.

Keywords: Müller cells, AFM, atomic force microscopy, retinoschisis, Notch

Sponsorship: This work was funded by a Herchel Smith Postdoctoral Fellowship to R.B.M., the Wellcome Trust programme in Developmental Biology to O.R. and J.O., NIH grants EY14358 (R.O.W.) and EY01730 (Vision Core), MRC Career Development Award and HFSP Young Investigator Grant to K.F., and a Wellcome Trust Investigator Award to W.A.H.


This record's URL: https://www.repository.cam.ac.uk/handle/1810/250389http://dx.doi.org/10.1083/jcb.201503115

Rights: Attribution-NonCommercial 2.0 UK: England & Wales

Licence URL: http://creativecommons.org/licenses/by-nc/2.0/uk/

Autor: MacDonald, Ryan B.Randlett, OwenOswald, JuliaYoshimatsu, TakeshiFranze, KristianHarris, William A.

Fuente: https://www.repository.cam.ac.uk/handle/1810/250389


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