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Müller glia provide essential tensile strength to the developing retina

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Publication Date: 2015-09-28

Journal Title: The Journal of Cell Biology

Publisher: The Rockefeller University Press

Volume: 210

Number: 7

Pages: 1075-1083

Language: English

Type: Article

Metadata: Show full item record

Citation: MacDonald, R., Randlett, O., Oswald, J., Yoshimatsu, T., Franze, K., & Harris, W. (2015). Müller glia provide essential tensile strength to the developing retina. The Journal of Cell Biology, 210 (7), 1075-1083.

Description: This is the final version of the article. It first appeared from the Rockefeller University Press via http://dx.doi.org/10.1083/jcb.201503115

Abstract: To investigate the cellular basis of tissue integrity in a vertebrate central nervous system (CNS) tissue, we eliminated Müller glial cells (MG) from the zebrafish retina. For well over a century, glial cells have been ascribed a mechanical role in the support of neural tissues, yet this idea has not been specifically tested in vivo. We report here that retinas devoid of MG rip apart, a defect known as retinoschisis. Using atomic force microscopy, we show that retinas without MG have decreased resistance to tensile stress and are softer than controls. Laser ablation of MG processes showed that these cells are under tension in the tissue. Thus, we propose that MG act like springs that hold the neural retina together, finally confirming an active mechanical role of glial cells in the CNS.

Sponsorship: We acknowledge R.O. Wong and E. Parker for electron microscopy assistance, K. O’Holleran and the Cambridge Advanced Imaging Centre (CAIC) for two-photon microscopy assistance, and A. Almeida for image analysis and careful reading of the manuscript. This work was funded by a Herchel Smith Postdoctoral Fellowship to R.B. MacDonald, the Wellcome Trust program in Developmental Biology to O. Randlett and J. Oswald, National Institutes of Health grants EY14358 (to R.O. Wong, University of Washington) and EY01730 (Vision Core), an Medical Research Council Career Development Award, a Human Frontier Science Program Young Investigator Grant to K. Franze, and a Wellcome Trust Investigator Award to W.A. Harris.


This record's URL: http://dx.doi.org/10.1083/jcb.201503115https://www.repository.cam.ac.uk/handle/1810/254032

Rights: Attribution-NonCommercial-ShareAlike 2.0 UK: England & Wales

Licence URL: http://creativecommons.org/licenses/by-nc-sa/2.0/uk/

Author: MacDonald, Ryan B.Randlett, Owen Oswald, Julia Yoshimatsu, Takeshi Franze, Kristian Harris, William A.

Source: https://www.repository.cam.ac.uk/handle/1810/254032


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