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Gain-of-function screen for genes that affect Drosophila muscle pattern formation.


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Publication Date: 2005-10-28

Journal Title: PLoS genetics

ISSN: 1553-7390

Volume: 1

Issue: 4

Pages: e55

Language: eng

Type: Article

Physical Medium: Print-Electronic

Metadata: Show full item record

Citation: Staudt, N., Molitor, A., Somogyi, K., Mata, J. I., Curado, S., Eulenberg, K., Meise, M., et al. (2005). Gain-of-function screen for genes that affect Drosophila muscle pattern formation

PLoS genetics, 1 (4), e55. https://doi.org/10.1371/journal.pgen.0010055

Abstract: This article reports the production of an EP-element insertion library with more than 3,700 unique target sites within the Drosophila melanogaster genome and its use to systematically identify genes that affect embryonic muscle pattern formation. We designed a UAS/GAL4 system to drive GAL4-responsive expression of the EP-targeted genes in developing apodeme cells to which migrating myotubes finally attach and in an intrasegmental pattern of cells that serve myotubes as a migration substrate on their way towards the apodemes. The results suggest that misexpression of more than 1.5% of the Drosophila genes can interfere with proper myotube guidance and/or muscle attachment. In addition to factors already known to participate in these processes, we identified a number of enzymes that participate in the synthesis or modification of protein carbohydrate side chains and in Ubiquitin modifications and/or the Ubiquitin-dependent degradation of proteins, suggesting that these processes are relevant for muscle pattern formation.

Keywords: Muscles, Cytoskeleton, Animals, Drosophila melanogaster, Drosophila Proteins, Ubiquitin, Genetic Techniques, Cell Cycle, Cell Movement, Gene Expression Regulation, Developmental, Body Patterning, Genes, Insect, Muscle Fibers, Skeletal

Identifiers:

External DOI: https://doi.org/10.1371/journal.pgen.0010055

This record's URL: https://www.repository.cam.ac.uk/handle/1810/265984



Rights: Attribution 4.0 International

Licence URL: http://creativecommons.org/licenses/by/4.0/





Autor: Staudt, NicoleMolitor, AndreasSomogyi, Kalman Mata, Juan IgnacioCurado, SilviaEulenberg, KarstenMeise, MartinSiegmund, ThomasHäde

Fuente: https://www.repository.cam.ac.uk/handle/1810/265984



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