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Analysis of replication factories in human cells by super-resolution light microscopy


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Publication Date: 2009-12-16

Language: English

Type: Article

Metadata: Show full item record

Citation: Cseresnyes, Z., Schwarz, U., & Green, C. M. (2009). Analysis of replication factories in human cells by super-resolution light microscopy.

Description: RIGHTS : This article is licensed under the BioMed Central licence at http://www.biomedcentral.com/about/license which is similar to the 'Creative Commons Attribution Licence'. In brief you may : copy, distribute, and display the work; make derivative works; or make commercial use of the work - under the following conditions: the original author must be given credit; for any reuse or distribution, it must be made clear to others what the license terms of this work are.

Abstract: Abstract Background DNA replication in human cells is performed in discrete sub-nuclear locations known as replication foci or factories. These factories form in the nucleus during S phase and are sites of DNA synthesis and high local concentrations of enzymes required for chromatin replication. Why these structures are required, and how they are organised internally has yet to be identified. It has been difficult to analyse the structure of these factories as they are small in size and thus below the resolution limit of the standard confocal microscope. We have used stimulated emission depletion (STED) microscopy, which improves on the resolving power of the confocal microscope, to probe the structure of these factories at sub-diffraction limit resolution. Results Using immunofluorescent imaging of PCNA (proliferating cell nuclear antigen) and RPA (replication protein A) we show that factories are smaller in size (approximately 150 nm diameter), and greater in number (up to 1400 in an early S- phase nucleus), than is determined by confocal imaging. The replication inhibitor hydroxyurea caused an approximately 40% reduction in number and a 30% increase in diameter of replication factories, changes that were not clearly identified by standard confocal imaging. Conclusions These measurements for replication factory size now approach the dimensions suggested by electron microscopy. This agreement between these two methods, that use very different sample preparation and imaging conditions, suggests that we have arrived at a true measurement for the size of these structures. The number of individual factories present in a single nucleus that we measure using this system is greater than has been previously reported. This analysis therefore suggests that each replication factory contains fewer active replication forks than previously envisaged.

Identifiers:

This record's URL: http://www.dspace.cam.ac.uk/handle/1810/237884http://dx.doi.org/10.1186/1471-2121-10-88

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Rights Holder: Cseresnyes et al.; licensee BioMed Central Ltd.





Autor: Cseresnyes, ZoltanSchwarz, UlfGreen, Catherine M.

Fuente: https://www.repository.cam.ac.uk/handle/1810/237884



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