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An analysis by metabolic labelling of the encephalomyocarditis virus ribosomal frameshifting efficiency and stimulators


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Publication Date: 2017-08-01

Journal Title: Journal of General Virology

Publisher: Society for General Microbiology

Volume: 98

Issue: 8

Pages: 2100-2105

Language: English

Type: Article

This Version: VoR

Metadata: Show full item record

Citation: Firth, A. E., & Ling, R. (2017). An analysis by metabolic labelling of the encephalomyocarditis virus ribosomal frameshifting efficiency and stimulators. Journal of General Virology, 98 (8), 2100-2105. https://doi.org/10.1099/jgv.0.000888

Abstract: Programmed −1 ribosomal frameshifting is a mechanism of gene expression whereby specific signals within messenger RNAs direct a proportion of ribosomes to shift −1 nt and continue translating in the new reading frame. Such frameshifting normally depends on an RNA structure stimulator 3$^\prime$-adjacent to a ‘slippery’ heptanucleotide shift site sequence. Recently we identified an unusual frameshifting mechanism in encephalomyocarditis virus, where the stimulator involves a $\textit{trans}$-acting virus protein. Thus, in contrast to other examples of −1 frameshifting, the efficiency of frameshifting in encephalomyocarditis virus is best studied in the context of virus infection. Here we use metabolic labelling to analyse the frameshifting efficiency of wild-type and mutant viruses. Confirming previous results, frameshifting depends on a G_GUU_UUU shift site sequence and a 3$^\prime$-adjacent stem-loop structure, but is not appreciably affected by the ‘StopGo’ sequence present ~30 nt upstream. At late timepoints, frameshifting was estimated to be 46–76 % efficient.

Keywords: gene expression, protein synthesis, translational control, ribosomal frameshifting, genetic recoding, virus

Sponsorship: Wellcome Trust [088789, 106207], UK Biotechnology and Biological Research Council (BBSRC) [BB/J007072/1], European Research Council (ERC) under the European Union’s Horizon 2020 research and innovation programme [grant agreement No (646891)].

Embargo Lift Date: 2100-01-01

Identifiers:

External DOI: https://doi.org/10.1099/jgv.0.000888

This record's URL: https://www.repository.cam.ac.uk/handle/1810/265723



Rights: Attribution 4.0 International

Licence URL: http://creativecommons.org/licenses/by/4.0/





Autor: Firth, Andrew EdwinLing, R

Fuente: https://www.repository.cam.ac.uk/handle/1810/265723



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