Vol 9: IgE Regulates the Expression of smMLCK in Human Airway Smooth Muscle Cells.Reportar como inadecuado



 Vol 9: IgE Regulates the Expression of smMLCK in Human Airway Smooth Muscle Cells.


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This article is from PLoS ONE, volume 9.AbstractPrevious studies have shown that enhanced accumulation of contractile proteins such as smooth muscle myosin light chain kinase smMLCK plays a major role in human airway smooth muscle cells HASM cell hypercontractility and hypertrophy. Furthermore, serum IgE levels play an important role in smooth muscle hyperreactivity. However, the effect of IgE on smMLCK expression has not been investigated. In this study, we demonstrate that IgE increases the expression of smMLCK at mRNA and protein levels. This effect was inhibited significantly with neutralizing abs directed against FcεRI but not with anti-FcεRII-CD23. Furthermore, Syk knock down and pharmacological inhibition of mitogen activated protein kinases MAPK ERK1-2, p38, and JNK and phosphatidylinositol 3-kinase PI3K significantly diminished the IgE-mediated upregulation of smMLCK expression in HASM cells. Taken together, our data suggest a role of IgE in regulating smMLCK in HASM cells. Therefore, targeting the FcεRI activation on HASM cells may offer a novel approach in controlling the bronchomotor tone in allergic asthma.



Autor: Balhara, Jyoti; Redhu, Naresh Singh; Shan, Lianyu; Gounni, Abdelilah S.

Fuente: https://archive.org/







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