Vol 9: Interaction between immobilized polyelectrolyte complex nanoparticles and human mesenchymal stromal cells.Reportar como inadecuado



 Vol 9: Interaction between immobilized polyelectrolyte complex nanoparticles and human mesenchymal stromal cells.


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This article is from International Journal of Nanomedicine, volume 9.AbstractBackground: Implant loosening or deficient osseointegration is a major problem in patients with systemic bone diseases eg, osteoporosis. For this reason, the stimulation of the regional cell population by local and sustained drug delivery at the bone-implant interface to induce the formation of a mechanical stable bone is promising. The purpose of this study was to investigate the interaction of polymer-based nanoparticles with human bone marrow-derived cells, considering nanoparticles’ composition and surface net charge. Materials and methods: Polyelectrolyte complex nanoparticles PECNPs composed of the polycations polyethyleneimine PEI, polyL-lysine PLL, or N,N-diethylaminoethyldextran DEAE in combination with the polyanions dextran sulfate DS or cellulose sulfate CS were prepared. PECNPs’ physicochemical properties size, net charge were characterized by dynamic light scattering and particle charge detector measurements. Biocompatibility was investigated using human mesenchymal stromal cells hMSCs cultured on immobilized PECNP films 5–50 nmol·cm−2 by analysis for metabolic activity of hMSCs in dependence of PECNP surface concentration by MTS 3-4,5-dimethylthiazol-2-yl-5-3-carboxymethoxyphenyl-2-4-sulfophenyl-2H-tet razolium, inner salt assay, as well as cell morphology phase contrast microscopy. Results: PECNPs ranging between ~50 nm and 150 nm were prepared. By varying the ratio of polycations and polyanions, PECNPs with a slightly positive PEC+NP or negative PEC−NP net charge were obtained. The PECNP composition significantly affected cell morphology and metabolic activity, whereas the net charge had a negligible influence. Therefore, we classified PECNPs into -variant systems- featuring a significant dose dependency of metabolic activity DEAE-CS, PEI-DS and -invariant systems- lacking such a dependency DEAE-DS, PEI-CS. Immunofluorescence imaging of fluorescein isothiocyanate isomer I FITC-labeled PECNPs suggested internalization into hMSCs remaining stable for 8 days. Conclusion: Our study demonstrated that PECNP composition affects hMSC behavior. In particular, the PEI-CS system showed biocompatibility in a wide concentration range, representing a suitable system for local drug delivery from PECNP-functionalized bone substitute materials.



Autor: Woltmann, Beatrice; Torger, Bernhard; Muller, Martin; Hempel, Ute

Fuente: https://archive.org/







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