Vol 6: Hydrogen atoms in protein structures: high-resolution X-ray diffraction structure of the DFPase.Reportar como inadecuado



 Vol 6: Hydrogen atoms in protein structures: high-resolution X-ray diffraction structure of the DFPase.


Vol 6: Hydrogen atoms in protein structures: high-resolution X-ray diffraction structure of the DFPase. - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Descargar gratis o leer online en formato PDF el libro: Vol 6: Hydrogen atoms in protein structures: high-resolution X-ray diffraction structure of the DFPase.
This article is from BMC Research Notes, volume 6.AbstractBackground: Hydrogen atoms represent about half of the total number of atoms in proteins and are often involved in substrate recognition and catalysis. Unfortunately, X-ray protein crystallography at usual resolution fails to access directly their positioning, mainly because light atoms display weak contributions to diffraction. However, sub-Ångstrom diffraction data, careful modeling and a proper refinement strategy can allow the positioning of a significant part of hydrogen atoms. Results: A comprehensive study on the X-ray structure of the diisopropyl-fluorophosphatase (DFPase) was performed, and the hydrogen atoms were modeled, including those of solvent molecules. This model was compared to the available neutron structure of DFPase, and differences in the protein and the active site solvation were noticed. Conclusions: A further examination of the DFPase X-ray structure provides substantial evidence about the presence of an activated water molecule that may constitute an interesting piece of information as regard to the enzymatic hydrolysis mechanism.



Autor: Elias, Mikael; Liebschner, Dorothee; Koepke, Jurgen; Lecomte, Claude; Guillot, Benoit; Jelsch, Christian; Chabriere, Eric

Fuente: https://archive.org/







Documentos relacionados