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 Vol 9: Heritable Multiplex Genetic Engineering in Rats Using CRISPR-Cas9.


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This article is from PLoS ONE, volume 9.AbstractThe CRISPR-Cas9 system has been proven to be an efficient gene-editing tool for genome modification of cells and organisms. Multiplex genetic engineering in rat holds a bright future for the study of complex disease. Here, we show that this system enables the simultaneous disruption of four genes (ApoE, B2m, Prf1, and Prkdc) in rats in one-step, by co-injection of Cas9 mRNA and sgRNAs into fertilized eggs. We further observed the gene modifications are germline transmittable, and confirmed the off-target mutagenesis and mosaicism are rarely detected by comprehensive analysis. Thus, the CRISPR-Cas9 system makes it possible to efficiently and reliably generate gene knock-out rats.



Autor: Ma, Yuanwu; Shen, Bin; Zhang, Xu; Lu, Yingdong; Chen, Wei; Ma, Jing; Huang, Xingxu; Zhang, Lianfeng

Fuente: https://archive.org/







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