Vol 15: Genome sequencing of high-penicillin producing industrial strain of Penicillium chrysogenum.Reportar como inadecuado



 Vol 15: Genome sequencing of high-penicillin producing industrial strain of Penicillium chrysogenum.


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This article is from BMC Genomics, volume 15.AbstractBackground: Due to the importance of Penicillium chrysogenum holding in medicine, the genome of low-penicillin producing laboratorial strain Wisconsin54-1255 had been sequenced and fully annotated. Through classical mutagenesis of Wisconsin54-1255, product titers and productivities of penicillin have dramatically increased, but what underlying genome structural variations is still little known. Therefore, genome sequencing of a high-penicillin producing industrial strain is very meaningful. Results: To reveal more insights into the genome structural variations of high-penicillin producing strain, we sequenced an industrial strain P. chrysogenum NCPC10086. By whole genome comparative analysis, we observed a large number of mutations, insertions and deletions, and structural variations. There are 69 new genes that not exist in the genome sequence of Wisconsin54-1255 and some of them are involved in energy metabolism, nitrogen metabolism and glutathione metabolism. Most importantly, we discovered a 53.7 Kb -new shift fragment- in a seven copies of determinative penicillin biosynthesis cluster in NCPC10086 and the arrangement type of amplified region is unique. Moreover, we presented two large-scale translocations in NCPC10086, containing genes involved energy, nitrogen metabolism and peroxysome pathway. At last, we found some non-synonymous mutations in the genes participating in homogentisate pathway or working as regulators of penicillin biosynthesis. Conclusions: We provided the first high-quality genome sequence of industrial high-penicillin strain of P. chrysogenum and carried out a comparative genome analysis with a low-producing experimental strain. The genomic variations we discovered are related with energy metabolism, nitrogen metabolism and so on. These findings demonstrate the potential information for insights into the high-penicillin yielding mechanism and metabolic engineering in the future. Electronic supplementary material: The online version of this article (doi:10.1186-1471-2164-15-S1-S11) contains supplementary material, which is available to authorized users.



Autor: Wang, Fu-Qiang; Zhong, Jun; Zhao, Ying; Xiao, Jingfa; Liu, Jing; Dai, Meng; Zheng, Guizhen; Zhang, Li; Yu, Jun; Wu, Jiayan; Duan, Baoling

Fuente: https://archive.org/







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