Potential Respiration in Oxyrrhis marina and Rhodomonas salinaReportar como inadecuado




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Director - Tutor: Packard, Theodore T

???metadata.dc.contributor.other???: Facultad de Ciencias del Mar

Materias : 251001 Oceanografía biológica

Fecha de publicación : 2009

Fecha de depósito: 24-ene-2013

Tipo de documento: Máster

Resumen : EN The Potential respiration in cultures of the heterotrophic flagellate Oxyrrhis marina (predator) and on amicro algae Rhodomonas salina (prey) was calculated from measurements of the electron transport system(ETS) activity. On R. salina these measurements were made during the exponential, stationary and celldeclinephase for a period of 64 days, but in O. marina they were only made during the period of starvation(cell-decline phase), for a period of 18 days. Time-courses of Chlorophyll, protein and cell density werecompared to the potential respiration (?). In R. salina the ??increased during the exponential phase inparallel with the cell- protein, cell number, and chlorophyll a. In O. marina, separated from its prey,??followed a similar pattern with the protein, chlorophyll and cell density. Flow cytometry proved useful notonly for cell-enumeration, but also for monitoring population changes in thenon-axenic mixed-populationcultures. From the physiological and biochemical measurements it is clear that the predator, O. marinawas metabolically more active than its prey, R. salina. Its ??was 19 nmO2 min-1 cell-1, 20 times higher thanthe ? of R. salina, with a ??of only 0,9 nmO2 min-1 cell-1. In addition O. marina was also 20 richer in proteinthan R. salina (942 versus 47 pg protein-cell). However its protein-specific ? was effectively the same (12versus 13 nmO2 min-1 (protein)-1, respectively. An interesting finding, that suggests that O. marina may beable to function as an autotroph, was the constancy, for 9 days, of the ingested chlorophyll-cell in O. marinadays after all its chlorophyll-rich prey was consumed.





Autor: Aristizábal, Manuela

Fuente: https://acceda.ulpgc.es


Introducción



Potential Respiration in Oxyrrhis marina  and Rhodomonas salina                                                   Tesis de Master   Manuela Aristizábal Bastidas  Director: Ted Packard  UNIVERSIDAD DE LAS PALMAS DE GRAN CANARIA  Programa de Master en Oceanografía  Las Palmas de Gran Canaria, España  Diciembre 18, 2009      Potential Respiration in Oxyrrhis marina and Rhodomonas salina Manuela Aristizábal Bastidas Universidad de Las Palmas de Gran Canaria   ABSTRACT The Potential respiration in cultures of the heterotrophic flagellate Oxyrrhis marina (predator) and on a micro algae Rhodomonas salina (prey) was calculated from measurements of the electron transport system (ETS) activity.
On R.
salina these measurements were made during the exponential, stationary and celldecline phase for a period of 64 days, but in O.
marina they were only made during the period of starvation (cell-decline phase), for a period of 18 days.
Time-courses of Chlorophyll, protein and cell density were compared to the potential respiration ().
In R.
salina the increased during the exponential phase in parallel with the cell- protein, cell number, and chlorophyll a.
In O.
marina, separated from its prey, followed a similar pattern with the protein, chlorophyll and cell density.
Flow cytometry proved useful not only for cell-enumeration, but also for monitoring population changes in thenon-axenic mixed-population cultures.
From the physiological and biochemical measurements it is clear that the predator, O.
marina was metabolically more active than its prey, R.
salina.
Its was 19 nmO2 min-1 cell-1, 20 times higher than the  of R.
salina, with a of only 0,9 nmO2 min-1 cell-1.
In addition O.
marina was also 20 richer in protein than R.
salina (942 versus 47 pg protein-cell).
However its protein-specific Φ was effectively the same (12 versus 13 nmO2 min-1 (protein)-1, r...






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