Identification of ATP synthase beta subunit ATPB on the cell surface as a non-small cell lung cancer NSCLC associated antigenReportar como inadecuado




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BMC Cancer

, 9:16

First Online: 14 January 2009Received: 27 May 2008Accepted: 14 January 2009DOI: 10.1186-1471-2407-9-16

Cite this article as: Lu, Z., Song, Q., Jiang, S. et al. BMC Cancer 2009 9: 16. doi:10.1186-1471-2407-9-16

Abstract

BackgroundAntibody-based immuneotherapy has achieved some success for cancer. But the main problem is that only a few tumor-associated antigens or therapeutic targets have been known to us so far. It is essential to identify more immunogenic antigens especially cellular membrane markers for tumor diagnosis and therapy.

MethodsThe membrane proteins of lung adenocarcinoma cell line A549 were used to immunize the BALB-c mice. A monoclonal antibody 4E7 McAb4E7 was produced with hybridoma technique. MTT cell proliferation assay was carried out to evaluate the inhibitory effect of McAb4E7 on A549 cells. Flow cytometric assay, immunohistochemistry, western blot and proteomic technologies based on 2-DE and mass spectrometry were employed to detect and identify the corresponding antigen of McAb4E7.

ResultsThe monoclonal antibody 4E7 McAb4E7 specific against A549 cells was produced, which exhibited inhibitory effect on the proliferation of A549 cells. By the proteomic technologies, we identified that ATP synthase beta subunit ATPB was the corresponding antigen of McAb4E7. Then, flow cytometric analysis demonstrated the localization of the targeting antigen of McAb4E7 was on the A549 cells surface. Furthermore, immunohistochemstry showed that the antigen of McAb4E7 mainly aberrantly expressed in tumor cellular membrane in non-small cell lung cancer NSCLC, but not in small cell lung cancer SCLC. The rate of ectopic expressed ATPB in the cellular membrane in lung adenocarcinoma, squamous carcinoma and their adjacent nontumourous lung tissues was 71.88%, 66.67% and 25.81% respectively.

ConclusionIn the present study, we identified that the ectopic ATPB in tumor cellular membrane was the non-small cell lung cancer NSCLC associated antigen. ATPB may be a potential biomarker and therapeutic target for the immunotherapy of NSCLC.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2407-9-16 contains supplementary material, which is available to authorized users.

Ze-jun Lu, Qi-fang Song contributed equally to this work.

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