Brefeldin A-inhibited guanine nucleotide-exchange protein 3 BIG3 is predicted to interact with its partner through an ARM-type α-helical structureReportar como inadecuado




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BMC Research Notes

, 7:435

Structural Biology

Abstract

BackgroundBrefeldin A-inhibited guanine nucleotide-exchange protein 3 BIG3 has been identified recently as a novel regulator of estrogen signalling in breast cancer cells. Despite being a potential target for new breast cancer treatment, its amino acid sequence suggests no association with any well-characterized protein family and provides little clues as to its molecular function. In this paper, we predicted the structure, function and interactions of BIG3 using a range of bioinformatic tools.

ResultsHomology search results showed that BIG3 had distinct features from its paralogues, BIG1 and BIG2, with a unique region between the two shared domains, Sec7 and DUF1981. Although BIG3 contains Sec7 domain, the lack of the conserved motif and the critical glutamate residue suggested no potential guaninyl-exchange factor GEF activity. Fold recognition tools predicted BIG3 to adopt an α-helical repeat structure similar to that of the armadillo ARM family. Using state-of-the-art methods, we predicted interaction sites between BIG3 and its partner PHB2.

ConclusionsThe combined results of the structure and interaction prediction led to a novel hypothesis that one of the predicted helices of BIG3 might play an important role in binding to PHB2 and thereby preventing its translocation to the nucleus. This hypothesis has been subsequently verified experimentally.

KeywordsBreast cancer Estrogen receptor-alpha BIG3 PHB2 Protein-protein interaction Bioinformatics Electronic supplementary materialThe online version of this article doi:10.1186-1756-0500-7-435 contains supplementary material, which is available to authorized users.

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Autor: Yi-An Chen - Yoichi Murakami - Shandar Ahmad - Tetsuro Yoshimaru - Toyomasa Katagiri - Kenji Mizuguchi

Fuente: https://link.springer.com/







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