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BMC Research Notes

, 1:100

First Online: 28 October 2008Received: 02 August 2008Accepted: 28 October 2008DOI: 10.1186-1756-0500-1-100

Cite this article as: Eilebrecht, S., Pellay, FX., Odenwälder, P. et al. BMC Res Notes 2008 1: 100. doi:10.1186-1756-0500-1-100

Abstract

BackgroundLittle is known about the physiological role of the EBER1 and 2 nuclear RNAs during Epstein Barr viral infection. The EBERs are transcribed by cellular RNA Polymerase III and their strong expression results in 10 to 10 copies per EBV infected cell, making them reliable diagnostic markers for the presence of EBV. Although the functions of most of the proteins targeted by EBER RNAs have been studied, the role of EBERs themselves still remains elusive.

FindingsThe cellular transcription response to EBER2 expression using the wild-type and an internal deletion mutant was determined. Significant changes in gene expression patterns were observed. A functional meta-analysis of the regulated genes points to inhibition of stress and immune responses, as well as activation of cellular growth and cytoskeletal reorganization as potential targets for EBER2 RNA. Different functions can be assigned to different parts of the RNA.

ConclusionThese results provide new avenues to the understanding of EBER2 and EBV biology, and set the grounds for a more in depth functional analysis of EBER2 using transcriptome activity measurements.

Sebastian Eilebrecht, François-Xavier Pellay contributed equally to this work.

Electronic supplementary materialThe online version of this article doi:10.1186-1756-0500-1-100 contains supplementary material, which is available to authorized users.

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Autor: Sebastian Eilebrecht - François-Xavier Pellay - Peter Odenwälder - Guillaume Brysbaert - Bernd-Joachim Benecke - Arndt Ben

Fuente: https://link.springer.com/



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