Mannosylated lipoarabinomannan in serum as a biomarker candidate for subclinical bovine tuberculosisReportar como inadecuado

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BMC Research Notes

, 7:559

Infectious Diseases


BackgroundEarly and unambiguous detection of bovine tuberculosis bTB, a significant disease of cattle worldwide, is necessary to control the spread of infection to other animals and humans. Current testing strategies are laborious, time consuming and heavily reliant on host responses that do not distinguish bTB from other mycobacteria. We report the presence of a pathogen signature, liparabinomannan LAM, as a potential biomarker for bTB infection.

FindingsFifty-five animals uninfected n = 33, bTb n = 10 and exposed cases n = 12 from a well characterized bovine serum repository were screened for the presence of LAM using a commercially available ELISA. Analysis showed that LAM had a sensitivity of 100% and a specificity of 91.7% for bTB detection bTB positive versus bTB exposed animals.

ConclusionLAM detection easily separated bTB infected animals from bTB exposed and negative controls. We propose that pathogen related markers, such as LAM, should be included with current testing strategies as a battery diagnostic for bTB.

KeywordsBovine tuberculosis Biomarker Mycobacterium bovis Cattle Lipoarabinomannan Diagnostics Subclinical Infection Serum AbbreviationsbTBBovine tuberculosis

CFTCaudal fold test


ManLAMMannosylated lipoarabionmannan

AraLAMNon-mannose-capped lipoarabinomannan

ELISAEnzyme-linked immunosorbent assay

ROCReceiver operating characteristic

AUCArea under the ROC curve

ODsOptical densities

HDLHigh density lipoprotein.

Electronic supplementary materialThe online version of this article doi:10.1186-1756-0500-7-559 contains supplementary material, which is available to authorized users.

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Autor: Elise A Lamont - João Ribeiro-Lima - Wade Ray Waters - Tyler Thacker - Srinand Sreevatsan


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