Role of B7-H4 siRNA in Proliferation, Migration, and Invasion of LOVO Colorectal Carcinoma Cell LineReportar como inadecuado




Role of B7-H4 siRNA in Proliferation, Migration, and Invasion of LOVO Colorectal Carcinoma Cell Line - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

BioMed Research International - Volume 2015 2015, Article ID 326981, 10 pages -

Research ArticleDigestive Endoscopy Center, Shanghai Tongren Hospital, Shanghai Jiao Tong University School of Medicine, 1111 Xianxia Road, Shanghai 200336, China

Received 7 December 2014; Accepted 3 April 2015

Academic Editor: Dominic Fan

Copyright © 2015 Hai-xia Peng et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objectives. Colorectal cancer is one of the most common malignancies. Recent studies investigated that B7-H4 is highly expressed in various cancers. We aimed at exploring the effect of B7-H4 siRNA on proliferation, invasion, and migration of LOVO cells which expressed B7-H4 notably. Design and Methods. Colon adenocarcinoma dataset was downloaded from The Cancer Genome Atlas. 35 colorectal cancer patients admitted to Shanghai Tongren Hospital were enrolled in this study. Cell proliferation and cell cycle distribution were identified by CCK8 and flow cytometry, respectively. Transwell assay was performed to detect the invasion and migration of LOVO cells. CXCL12-CXCR4 expression and JAK2-STAT3 phosphorylation were determined by real-time PCR and western blot. Results. B7-H4 expressed is elevated in colorectal cancer tissues than in the adjacent normal tissues. B7-H4 siRNA effectively inhibited the proliferation at 24 h and 48 h, arrested cell cycle at G0-G1, and suppressed cell invasion and migration. Gene set enrichment analysis showed that CXCL12-CXCR4 and JAK-STAT were correlative with the B7-H4 expression. Additionally, CXCL12-CXCR4 expression and JAK2-STAT3 phosphorylation were reduced. Conclusions. B7-H4 siRNA can effectively inhibit proliferation, invasion, and migration of LOVO cells by targeting CXCL12-CXCR4 and JAK2-STAT3 signaling, which can serve as a new target for colorectal carcinoma treatment.





Autor: Hai-xia Peng, Wei-qi Wu, Da-ming Yang, Rong Jing, Ji Li, Feng-li Zhou, Yun-fei Jin, Sai-yu Wang, and Yi-min Chu

Fuente: https://www.hindawi.com/



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