Modeling interactions between Human Equilibrative Nucleoside Transporter-1 and other factors involved in the response to gemcitabine treatment to predict clinical outcomes in pancreatic ductal adenocarcinoma patientsReportar como inadecuado




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Journal of Translational Medicine

, 12:248

Computational Modelling and Epidemiology

Abstract

BackgroundPancreatic ductal adenocarcinoma PDAC is an extremely aggressive malignancy, characterized by largely unsatisfactory responses to the currently available therapeutic strategies. In this study we evaluated the expression of genes involved in gemcitabine uptake in a selected cohort of patients with PDAC, with well-defined clinical-pathological features.

MethodsmRNA levels of hENT1, CHOP, MRP1 and DCK were evaluated by means of qRT-PCR in matched pairs of tumor and adjacent normal tissue samples collected from PDAC patients treated with gemcitabine after surgical tumor resection. To detect possible interaction between gene expression levels and to identify subgroups of patients at different mortality-progression risk, the RECursive Partitioning and Amalgamation RECPAM method was used.

ResultsRECPAM analysis showed that DCK and CHOP were most relevant variables for the identification of patients with different mortality risk, while hENT1 and CHOP were able to identify subgroups of patients with different disease progression risk. Conclusion: hENT1, CHOP, MRP1 and DCK appear correlated to PDAC, and this interaction might influence disease behavior.

KeywordsPancreatic ductal adenocarcinoma hENT1 CHOP MRP1 DCK RECPAM Electronic supplementary materialThe online version of this article doi:10.1186-s12967-014-0248-4 contains supplementary material, which is available to authorized users.

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Autor: Francesca Tavano - Andrea Fontana - Fabio Pellegrini - Francesca Paola Burbaci - Francesca Rappa - Francesco Cappello - Mas

Fuente: https://link.springer.com/



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