Formation of posterior cranial placode derivatives requires the Iroquois transcription factor irx4aReportar como inadecuado




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Resumen

Members of the Iroquois Irx homeodomain transcription factor gene family have been implicated in avariety of early developmental processes, including neural pre-patterning, tissue differentiation, neural crestdevelopment and cranial placode formation. Here, we report that, in zebrafish, the irx4a gene participates inspecification of a number of placode derivatives that arise from the posterior placodal field. Specifically,differentiation of the trigeminal, epibranchial and lateral line placodes are affected when irx4a function isinterrupted using antisense morpholino oligonucleotides. We show that both in the trigeminal ganglion andin the lateral line, irx4a is involved in controlling the number of sensory cells that develop. Other phenotypesobserved in morphant embryos include misspecification of the heart chambers and failure of retinal ganglionand photoreceptor cell differentiation, functions described previously for Irx4 in other species. We alsoprovide evidence that irx4a regulates the expression of the sox2 gene, both in the neural plate and inprogenitor cells of the lateral line system. Our results point to irx4a as a critical gene for numerousdevelopmental processes and highlight its role in the formation of placodal derivatives in vertebrates.



Autor: Feijóo, Carmen Gloria; - Saldías, Marioli P.; - Paz, Javiera F. de la; - Gómez Skarmeta, José Luis; - Allende Connelly, Migue

Fuente: http://repositorio.uchile.cl/



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