Expression of transcript factors SALL4 and OCT4 in a subset of non-small cell lung carcinomas NSCLCReportar como inadecuado

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Translational Respiratory Medicine

, 2:10

First Online: 02 October 2014Received: 05 August 2014Accepted: 20 August 2014DOI: 10.1186-s40247-014-0010-7

Cite this article as: Rodriguez, E., Chen, L., Ao, MH. et al. Transl Respir Med 2014 2: 10. doi:10.1186-s40247-014-0010-7


BackgroundSALL4 and OCT4 are transcription factors and play essential roles in stem cell development and oncogenesis. However, the expression of these transcription factors has not been well studied in lung cancers. In this study, we evaluated the expression of SALL4 and OCT4 in non-small cell lung carcinomas NSCLC by immunochemistry. NSCLC tissue microarrays TMAs were constructed with a total of 77 primary lung adenocarcinomas ADCs and 90 primary lung squamous cell carcinomas SqCCs. A mouse monoclonal anti-human SALL4 1:400 dilution and a polyclonal anti-human OCT4 1:200 dilution antibodies were used. Nuclear staining of SALL4 and OCT4 was scored semi-quantitatively using a three tiered scale. The expressions of SALL4 and OCT4 were correlated with the tumor differentiation, pathological stage, and patients- clinical information.

ResultsIn primary ADCs, the stronger expression of SALL4 and OCT4 was 7.8% and 9.1%, respectively. The stronger expression of SALL4 was inversely correlated with tumor differentiations. In primary SqCCs, the stronger expressions of SALL4 and OCT4 were 16.7% and 0%, respectively. The expression of SALL4 is correlated with the expression of OCT4, but inversely correlated with the tumor stage in SqCCs.

ConclusionsWe found that both SALL4 and OCT4 were differentially expressed in a subset of primary ADC and SqCC. Our finding suggest that different stem cell markers may be expressed and-or play differential role in different subtypes of NSCLC. The potential role of SALL4 and OCT4 needs to be further investigated in NSCLC.

KeywordsTranscription factor SALL4 and OCT4 Immunohistochemistry IHC Non-small cell lung cancer NSCLC Metastatic NSCLC Lung cancer biopsy and tissue microarray TMA Electronic supplementary materialThe online version of this article doi:10.1186-s40247-014-0010-7 contains supplementary material, which is available to authorized users.

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Autor: Erika Rodriguez - Li Chen - Ming-Hui Ao - Susan Geddes - Ed Gabrielson - Frederic Askin - Hui Zhang - Qing Kay Li


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