CNBP mediates neural crest cell expansion by controlling cell proliferation and cell survival during rostral head developmentReportar como inadecuado




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Resumen

Striking conservation in various organisms suggests that cellular nucleic acid binding protein CNBPplays a fundamental biological role across different species. Recently, it was reported that CNBP is required for forebrainformation during chick and mouse embryogenesis. In this study, we have used the zebrafish model system to expandand contextualize the basic understanding of the molecular mechanisms of CNBP activity during vertebrate headdevelopment. We show that zebrafish cnbp is expressed in the anterior CNS in a similar fashion as has been observed inearly chick and mouse embryos. Using antisense morpholino oligonucleotide knockdown assays, we show that CNBPdepletion causes forebrain truncation while trunk development appears normal. A substantial reduction in cellproliferation and an increase in cell death were observed in the anterior regions of cnbp morphant embryos, mainlywithin the cnbp expression territory. In situ hybridization assays show that CNBP depletion does not affect CNS patterningwhile it does cause depletion of neural crest derivatives. Our data suggest an essential role for CNBP in mediatingneural crest expansion by controlling proliferation and cell survival rather than via a cell fate switch during rostral headdevelopment. This possible role of CNBP may not only explain the craniofacial anomalies observed in zebrafish but alsothose reported for mice and chicken and, moreover, demonstrates that CNBP plays an essential and conserved role duringvertebrate head development.



Autor: Weiner, Andrea; - Allende Connelly, Miguel; - Becker, T. S.; - Calcaterra, Nora B.; -

Fuente: http://repositorio.uchile.cl/



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