Intraperitoneal Exposure to Nano-Microparticles of Fullerene Increases Acetylcholinesterase Activity and Lipid Peroxidation in Adult Zebrafish Danio rerio BrainReportar como inadecuado




Intraperitoneal Exposure to Nano-Microparticles of Fullerene Increases Acetylcholinesterase Activity and Lipid Peroxidation in Adult Zebrafish Danio rerio Brain - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

BioMed Research InternationalVolume 2013 2013, Article ID 623789, 11 pages

Research Article

Laboratório de Biologia Genômica e Molecular, Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul, Avenida Ipiranga 6681 Sala 134, 90619-900 Porto Alegre, RS, Brazil

Instituto Nacional de Ciência e Tecnologia Translacional em Medicina INCT-TM, 90035-003 Porto Alegre, RS, Brazil

Universidade Federal do Rio Grande-FURG, Instituto de Ciências Biológicas ICB, Avenida Itália Km 8 s-n, 96208-900 Rio Grande, RS, Brazil

Programa de Pós-Graduação em Ciências Fisiológicas-Fisiologia Animal Comparada, FURG, Avenida Itália Km 8 s-n, 96208-900 Rio Grande, RS, Brazil

Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Avenida Ipiranga 2752, 90610-000 Porto Alegre, RS, Brazil

Laboratório de Neuroquímica e Psicofarmacologia, Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul, Avenida Ipiranga 6681, 90619-900 Porto Alegre, RS, Brazil

Instituto Nacional de Ciência e Tecnologia de Nanomateriais de Carbono, 31270-901 Belo Horizonte, MG, Brazil

Received 5 April 2013; Accepted 29 May 2013

Academic Editor: Qaisar Mahmood

Copyright © 2013 Gonzalo Ogliari Dal Forno et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Even though technologies involving nano-microparticles have great potential, it is crucial to determine possible toxicity of these technological products before extensive use. Fullerenes C60 are nanomaterials with unique physicochemical and biological properties that are important for the development of many technological applications. The aim of this study was to evaluate the consequences of nonphotoexcited fullerene C60 exposure in brain acetylcholinesterase expression and activity, antioxidant responses, and oxidative damage using adult zebrafish as an animal model. None of the doses tested 7.5, 15, and 30 mg-kg altered AChE activity, antioxidant responses, and oxidative damage when zebrafish were exposed to nonphotoexcited C60 nano-microparticles during 6 and 12 hours. However, adult zebrafish exposed to the 30 mg-kg dose for 24 hours have shown enhanced AChE activity and augmented lipid peroxidation TBARS assays in brain. In addition, the up-regulation of brain AChE activity was neither related to the transcriptional control RT-qPCR analysis nor to the direct action of nonphotoexcited C60 nano-microparticles on the protein in vitro results but probably involved a posttranscriptional or posttranslational modulation of this enzymatic activity. Taken together these findings provided further evidence of toxic effects on brain after C60 exposure.





Autor: Gonzalo Ogliari Dal Forno, Luiza Wilges Kist, Mariana Barbieri de Azevedo, Rachel Seemann Fritsch, Talita Carneiro Brandão Pe

Fuente: https://www.hindawi.com/



DESCARGAR PDF




Documentos relacionados