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BMC Research Notes

, 7:766

Developmental Biology

Abstract

BackgroundThe central nervous system has a complex structural organization and consists of different subdomains along the antero-posterior axis. However, questions remain about the molecular mechanisms leading to the regionalization of this organ. We used a previously developed methodology to identify the novel patterning role of GDF11, a TGF-β signaling factor.

FindingsUsing an assay based on neural differentiated mouse embryonic stem cells, GDF11 is shown to induce diencephalic posterior forebrain, mesencephalic midbrain and metencephalic anterior hindbrain fates at the expense of telencephalic anterior forebrain specification. GDF11 has not previously been implicated in the early patterning of the nervous system. In addition, inhibition of the TGF-β type I receptors Alk4, Alk5 and Alk7 by the pharmacological inhibitor SB431542 caused a strong anteriorization of the cells.

ConclusionsOur findings suggest that GDF11 is involved in the earliest steps of the brain patterning during neurogenesis in the vertebrate embryo and is shown to be a regionalizing factor of the regional fate in the developing brain. This regionalization is not a typical posteriorizing signal as seen with retinoic acid, FGF or BMP molecules. To our knowledge, this is the first time that GDF11 is implicated in the earliest steps of the patterning of the neural plate.

KeywordsMouse ES cells Brain patterning GDF11 Electronic supplementary materialThe online version of this article doi:10.1186-1756-0500-7-766 contains supplementary material, which is available to authorized users.

Nele Vanbekbergen, Marijke Hendrickx contributed equally to this work.

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Autor: Nele Vanbekbergen - Marijke Hendrickx - Luc Leyns

Fuente: https://link.springer.com/







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