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BMC Research Notes

, 7:846

Plant Biology

Abstract

BackgroundOrdered collections of mutants serve as invaluable tools in biological research. TILLING Targeting Induced Local Lesions IN Genomes provides an efficient method to discover, in mutagenized populations, the possible phenotypes controlled by gene sequences whose function is unknown. This method can replace transgenic techniques for the functional validation of cloned genes, especially in the case of transformation-recalcitrant plants such as cucumber.

ResultsWe report the development of a TILLING cucumber population, generated by EMS mutagenesis in the Poinsett76 genetic background. The population was evaluated by screening for morphological mutations, and a range of developmental, pigmentation and spontaneous lesion mutants were observed. Suitability for detecting single nucleotide polymorphism in selected genes has been tested by screening a sample of amplicons, with detection rate of 1 SNP in ~1 Mbp.

ConclusionThe population described in this Research Note represents a useful asset in cucumber research, to be exploited for forward genetic screens and functional genomics purposes.

KeywordsCucumis sativus EMS mutagenesis Mutant screen TILLING AbbreviationsCAPSCleaved amplified polymorphic sequence

EMSEthyl methanesulfonate

PCRPolymerase chain reaction

SNPSingle nucleotide polymorphism

TILLINGTargeting Induced Local Lesions IN Genomes.

Electronic supplementary materialThe online version of this article doi:10.1186-1756-0500-7-846 contains supplementary material, which is available to authorized users.

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Autor: Rina Fraenkel - Irina Kovalski - Christelle Troadec - Abdelhafid Bendahmane - Rafael Perl-Treves

Fuente: https://link.springer.com/







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