Prognostic influence of cyclooxygenase-2 protein and mRNA expression in node-negative breast cancer patientsReportar como inadecuado

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BMC Cancer

, 14:952

Translational oncology


BackgroundCyclooxygenases COX play a key role in prostaglandin metabolism and are important for tumor development and progression. The aim of this study was to analyze the prognostic impact of COX-2 expression in a cohort of lymph node-negative breast cancer patients not treated in the adjuvant setting.

MethodsCOX-2 expression was determined by immunohistochemistry IHC in tumor tissue of 193 node-negative breast cancer patients. Additionally, mRNA expression was determined in corresponding tumor samples using microarray based gene-expression data. Univariate and multivariate Cox regression analyses adjusted for age at diagnosis, tumor size, histological grade, human epithelial growth factor receptor 2 HER2, estrogen receptor ER and progesterone receptor PR were performed to evaluate the association of both COX-2 protein and mRNA expression with survival. Survival rates were determined by the Kaplan-Meier method. Correlations between COX-2 expression and established prognostic factors were analyzed using the Chi-square test. A potential correlation between COX-2 protein expression and COX-2 mRNA expression was assessed utilizing the Kruscal-Wallis-H-test.

ResultsCOX-2 protein expression was positive in 24.9% of the breast cancer samples. Univariate analysis showed that COX-2 protein expression was associated with shorter disease-free survival DFS P = 0.0001, metastasis-free survival MFS P = 0.002 as well as breast cancer specific overall survival OS P = 0.043. In multivariate analysis COX-2 expression retained its significance independent of established prognostic factors for shorter DFS P < 0.001, HR = 2.767, 95% CI = 1.563-4.901 and for inferior MFS P = 0.002, HR = 2.7, 95% CI = 1.469-5.263 but not for OS P = 0.096, HR = 1.929, 95% CI = 0.889-4.187. In contrast, COX-2 mRNA expression was not related to survival and failed to show a correlation with protein expression P = 0.410.

ConclusionsThe present findings support the hypothesis that COX-2 protein but not mRNA expression is associated with an unfavorable outcome in node-negative breast cancer.

KeywordsCOX-2 Breast cancer Node-negative Prognosis Electronic supplementary materialThe online version of this article doi:10.1186-1471-2407-14-952 contains supplementary material, which is available to authorized users.

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Autor: Isabel Sicking - Karlien Rommens - Marco J Battista - Daniel Böhm - Susanne Gebhard - Antje Lebrecht - Cristina Cotarelo


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