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Journal of Translational Medicine

, 6:39

First Online: 22 July 2008Received: 23 June 2008Accepted: 22 July 2008DOI: 10.1186-1479-5876-6-39

Cite this article as: Jin, P., Wang, E., Ren, J. et al. J Transl Med 2008 6: 39. doi:10.1186-1479-5876-6-39


BackgroundMobilized-peripheral blood hematopoietic stem cells HSCs have been used for transplantation, immunotherapy, and cardiovascular regenerative medicine. Agents used for HSC mobilization include G-CSF and the CXCR4 inhibitor AMD3100 plerixafor. The HSCs cells mobilized by each agent may contain different subtypes and have different functions. To characterize mobilized HSCs used for clinical applications, microRNA miRNA profiling and gene expression profiling were used to compare AMD3100-mobilized CD133+ cells from 4 subjects, AMD3100 plus G-CSF-mobilized CD133+ cells from 4 subjects and G-CSF-mobilized CD34+ cells from 5 subjects. The HSCs were compared to peripheral blood leukocytes PBLs from 7 subjects.

ResultsHierarchical clustering of miRNAs separated HSCs from PBLs. miRNAs up-regulated in all HSCs included hematopoiesis-associated miRNA; miR-126, miR-10a, miR-221 and miR-17-92 cluster. miRNAs up-regulated in PBLs included miR-142-3p -218 -21, and -379. Hierarchical clustering analysis of miRNA expression separated the AMD3100-mobilized CD133+ cells from G-CSF-mobilized CD34+ cells. Gene expression analysis of the HSCs naturally segregated samples according to mobilization and isolation protocol and cell differentiation status.

ConclusionHSCs and PBLs have unique miRNA and gene expression profiles. miRNA and gene expression microarrays maybe useful for assessing differences in HSCs.

Electronic supplementary materialThe online version of this article doi:10.1186-1479-5876-6-39 contains supplementary material, which is available to authorized users.

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Autor: Ping Jin - Ena Wang - Jiaqiang Ren - Richard Childs - Jeong Won Shin - Hanh Khuu - Francesco M Marincola - David F Stro

Fuente: https://link.springer.com/

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