Choline and Cystine Deficient Diets in Animal Models with Hepatocellular Injury: Evaluation of Oxidative Stress and Expression of RAGE, TNF-α, and IL-1βReportar como inadecuado




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Oxidative Medicine and Cellular Longevity - Volume 2015 2015, Article ID 121925, 11 pages -

Research Article

Faculdade de Nutrição, Universidade Federal de Alagoas UFAL, Campus A. C. Simões, Avenida Lourival Melo Mota, S-N, Tabuleiro dos Martins, 57072-970 Maceió, AL, Brazil

Instituto de Química e Biotecnologia IQB, Universidade Federal de Alagoas UFAL, Avenida Lourival Melo Mota, s-n, Cidade Universitária, 57072-900 Maceió, AL, Brazil

Instituto Federal de Educação, Ciência e Tecnologia de Alagoas, 57020-600 Maceió, AL, Brazil

Programa de Pós Graduação em Ciências da Saúde PPGCS, Universidade Federal de Alagoas UFAL, Campus A. C. Simões, Avenida Lourival Melo Mota, s-n, Tabuleiro dos Martins, 57072-970 Maceió, AL, Brazil

Instituto de Ciências Biológicas e da Saúde, Universidade Federal de Alagoas, 57072-900 Maceió, AL, Brazil

Centro de Estudos em Estresse Oxidativo CEEO, Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde ICBS, Universidade Federal do Rio Grande do Sul UFRGS, Rua Ramiro Barcelos 2600 Anexo, Santana, 90035003 Porto Alegre, RS, Brazil

INCT de Bioanalítica, UNICAMP, 13083-861 Campinas, SP, Brazil

Received 17 November 2014; Revised 15 May 2015; Accepted 18 May 2015

Academic Editor: Matthew C. Zimmerman

Copyright © 2015 Juliana Célia F. Santos et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study aims to evaluate the effects of diets deficient in choline and-or cystine on hepatocellular injury in animal models young male Wistar rats, aged 21 days, by monitoring some of the oxidative stress biomarkers and the expression of RAGE, TNF-α, and IL-1β. The animals were divided into 6 groups and submitted to different diets over 30 days: AIN-93 diet standard, St, AIN-93 choline deficient CD diet and AIN-93 choline and cystine deficient CCD diet, in the pellet pl and powder pw diet forms. Independently of the diet form, AIN-93 diet already led to hepatic steatosis and CD-CCD diets provoked hepatic damage. The increase of lipid peroxidation, represented by the evaluation of thiobarbituric acid reactive species, associated with the decrease of levels of antioxidant enzymes, were the parameters with higher significance toward redox profile in this model of hepatic injury. Regarding inflammation, in relation to TNF-α, higher levels were evidenced in , while, for IL-1β, no significant alteration was detected. RAGE expression was practically the same in all groups, with exception of versus . These results together confirm that AIN-93 causes hepatic steatosis and choline and-or cysteine deficiencies produce important hepatic injury associated with oxidative stress and inflammatory profiles.





Autor: Juliana Célia F. Santos, Orlando R. P. de Araújo, Iara B. Valentim, Kívia Queiroz de Andrade, Fabiana Andréa Moura, Salet

Fuente: https://www.hindawi.com/



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