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BMC Research Notes

, 1:22

First Online: 05 June 2008Received: 01 February 2008Accepted: 05 June 2008DOI: 10.1186-1756-0500-1-22

Cite this article as: Chalam, K.V., Sharma, R.K. & Murthy, R.K. BMC Res Notes 2008 1: 22. doi:10.1186-1756-0500-1-22


BackgroundNerve growth factor NGF helps in the healing and survival of ganglion cells, photoreceptors, and optic nerve after injury and has been implicated to have a role in pathophysiology of glaucoma. So far, in animal studies, injury to iris in vitro has revealed an increase in NGF levels in aqueous. There is a great interest in investigating the levels of NGF in human aqueous in glaucomatous eyes, as suggested by animal studies, to gain a better understanding of the pathophysiology of glaucoma.

FindingsIn this study, we examined the presence of NGF levels in aqueous humor collected from human eyes and the limitations in determining the NGF levels in human samples. NGF was assessed by ELISA immunoassay in undiluted aqueous samples collected from 32 consecutive patients undergoing surgery for cataract control or primary open angle glaucoma POAG. Recombinant NGF was used as positive control. NGF levels were below undetectable levels in aqueous humor from eyes with POAG and controls by immunoassay. Less than 10% of samples had detectable NGF levels and these were considered outliers.

ConclusionOur result highlights the undetectable levels of NGF in human aqueous samples.

Electronic supplementary materialThe online version of this article doi:10.1186-1756-0500-1-22 contains supplementary material, which is available to authorized users.

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Autor: Kakarla V Chalam - Rajesh K Sharma - Ravi K Murthy


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