MicroRNA-572 expression in multiple sclerosis patients with different patterns of clinical progressionReportar como inadecuado




MicroRNA-572 expression in multiple sclerosis patients with different patterns of clinical progression - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Journal of Translational Medicine

, 13:148

First Online: 07 May 2015Received: 12 November 2014Accepted: 24 April 2015DOI: 10.1186-s12967-015-0504-2

Cite this article as: Mancuso, R., Hernis, A., Agostini, S. et al. J Transl Med 2015 13: 148. doi:10.1186-s12967-015-0504-2

Abstract

BackgroundDemyelination and failure of remyelination are core mechanisms in the pathogenesis of multiple sclerosis MS; the factors modulating these processes are still mostly unknown. MicroRNA 572 miR-572 is deregulated in MS and is suggested to targets neural cell adhesion molecule NCAM, a glycoprotein involved in CNS reparative mechanisms. The aim of this study is to analyze miR-572 in patients with different clinical phenotypes of MS.

MethodsqPCR quantification of miR-572 isolated from serum was performed in 16 primary progressive PP, 15 secondary progressive SP, 31 relapsing remitting RR MS patients and 15 sex-and age-matched healthy controls.

ResultsmiR-572 expression was reduced overall in MS patients p < 0.05 compared to HC; this miRNA was significantly upregulated in SPMS and in RRMS during disease relapse, whereas it was downregulated in PPMS and in quiescent phases of RRMS. miR-572 expression correlated with EDSS scores RSp = 0.491; p < 0.05 independently of the clinical phenotype. The results suggest that this miRNA might be a tool that helps distinguishing between PPMS and SPMS and between relapsing and remitting phases in RRMS.

ConclusionsEvaluation of miR-572 may serve as a non-invasive biomarker for remyelination.

KeywordsmicroRNA Multiple Sclerosis NCAM Remyelination Disability Serum AbbreviationsANOVAOne-way analysis of variance

AUCArea under curve

cDNAComplementary DNA

CIConfidence interval

CNSCentral nervous system

CqQuantification cycle

CSFCerebrospinal fluid

EDSSKurtzke Expanded Disability Status Scale

HCHealthy control

LNALocked nucleic acid

miRNAmicroRNAs

MRIMagnetic resonance imaging

MSMultiple Sclerosis

NCAMNeural cell adhesion molecule

NCAMsSoluble NCAM

OPCOligodendrocytes precursor cells

PPMSPrimary progressive MS

PSA-NCAMPolysialylated NCAM

qPCRQuantitative polymerase chain reaction

ROCReceiver operating characteristics analysis

RRMSRelapsing remitting MS

SPMSSecondary progressive MS

Electronic supplementary materialThe online version of this article doi:10.1186-s12967-015-0504-2 contains supplementary material, which is available to authorized users.

Download fulltext PDF



Autor: Roberta Mancuso - Ambra Hernis - Simone Agostini - Marco Rovaris - Domenico Caputo - Mario Clerici

Fuente: https://link.springer.com/







Documentos relacionados