Evaluation of in vitro anti-inflammatory and antibacterial potential of Crescentia cujete leaves and stem barkReportar como inadecuado




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BMC Research Notes

, 8:412

Pharmacology andToxicology

Abstract

BackgroundThe various parts of Cresecentia cujete have some important biological activities. In folklore medicine leaves are used to treat hematomas, tumors and hypertension. Fruit decoction is used to treat diarrhea, stomachaches, cold, bronchitis, cough, asthma, and urethritis. The present study was designed to explore the anti-inflammatory and antibacterial potential of C. cujete leaves and stem bark. Anti-inflammatory activity was evaluated by in vitro human red blood cell HRBC membrane stabilization method and antibacterial activity by disc diffusion method.

MethodsIn vitro anti-inflammatory activity was evaluated by human red blood cell HRBC membrane stabilization method while in vitro antibacterial activity was evaluated using cultures of Escherichia coli and Staphylococcus aureus by disc diffusion method. Total phenolic TPC and total flavonoid contents TFC of the crude extract and fractions were also determined by Folin–Ciocalteu’s phenol reagent and by aluminium chloride method, respectively.

ResultsThe crude ethanol extract CEE of leaves and bark concentration of each 1.0 mg-ml demonstrated strong membrane stabilizing activity 53.86 and 61.85 % protection, respectively, whereas their chloroform fractions CHF revealed moderate activity 48.74 ± 0.56 and 43.55 ± 6.20 %, respectively compared with standard aspirin concentration 0.10 mg-ml which showed 75.81 % protection in this test. All the samples showed a dose dependent anti-inflammatory activity in HRBC membrane stabilization test. Total phenolic TPC and total flavonoid contents TFC of the crude extract and fractions were also determined. Again, in in vitro antibacterial study, the extractives exhibited potent antibacterial activity.

ConclusionResults from this study showed that the leaves and bark of C. cujete possessed anti-inflammatory as well as antibacterial activities indicating that the plant extract has therapeutic potential against the bacterial infection and also have effect on disease processes by causing destabilization of biological membranes.

KeywordsAnti-inflammatory Antibacterial Total phenol Leaves Stem bark AbbreviationsC. cujeteCresecentia cujete

HRBChuman red blood cell

TPCtotal phenolic and

TFCtotal flavonoid contents

CEEcrude ethanol extract

CHFchloroform fractions

NSAIDsnon-steroidal anti-inflammatory drugs

DPPH2,2-diphenyl-1-picrylhydrazyl

CAcatechin

AAascorbic acid

TCAtrichloro acetic acid

EDTAethylenediamine tetra acetic acid

GAEgallic acid equivalent

QEQuercetin equivalent

NSno sensitivity

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Autor: Mst. Shahnaj Parvin - Nandita Das - Nusrat Jahan - Most. Afia Akhter - Laizuman Nahar - Md. Ekramul Islam

Fuente: https://link.springer.com/







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