Comparative Cytogenetics Analysis of Chlamys farreri, Patinopecten yessoensis, and Argopecten irradians with C0t-1 DNA by Fluorescence In Situ HybridizationReportar como inadecuado




Comparative Cytogenetics Analysis of Chlamys farreri, Patinopecten yessoensis, and Argopecten irradians with C0t-1 DNA by Fluorescence In Situ Hybridization - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Evidence-Based Complementary and Alternative MedicineVolume 2011 2011, Article ID 785831, 7 pages

Research ArticleKey Laboratory of Marine Genetics and Breeding MGB, College of Marine Life Sciences, Ocean University of China, Ministry of Education, Qingdao 266003, China

Received 12 January 2011; Accepted 16 May 2011

Copyright © 2011 Li-Ping Hu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The chromosomes of Chlamys farreri, Patinopecten yessoensis, and Argopecten irradians were studied by FISH using C. farreriC0t-1 DNA probes. The results showed that C0t-1 DNA signals spread on all chromosomes in the three scallops, whereas signal density and intensity were different strikingly. Clustering brighter signals presented in the centromeric and telomeric regions of most C. farreri chromosomes, and in the centromeric or pericentromeric regions of several P. yessoensis chromosomes. Comparative analysis of the mapping indicated a relatively higher homology in the repetitive DNA sequences of the genome between C. farreri and P. yessoensis than that between C. farreri and A. irradians. In addition, FISH showed that the distribution of C0t-1 DNA clustering signals in C. farreri displayed completely similar signal bands between homologous chromosomes. Based on the C0t-1 DNA fluorescent bands, a more exact karyotype of C. farreri has been obtained. In this study, the comparative analysis based on C0t-1 DNA provides a new insight into the chromosomal reconstructions during the evolution process, and it is helpful for understanding an important source of genomic diversity, species relationships, and genome evolution.





Autor: Li-Ping Hu, Wen-Cong Shang, Yan Sun, Shan Wang, Xiao-Liang Ren, Xiao-Ting Huang, and Zhen-Min Bao

Fuente: https://www.hindawi.com/



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