LncRNA-GAS5 induces PTEN expression through inhibiting miR-103 in endometrial cancer cellsReportar como inadecuado




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Journal of Biomedical Science

, 22:100

First Online: 29 October 2015Received: 16 June 2015Accepted: 16 October 2015DOI: 10.1186-s12929-015-0213-4

Cite this article as: Guo, C., Song, W., Sun, P. et al. J Biomed Sci 2015 22: 100. doi:10.1186-s12929-015-0213-4

Abstract

BackgroundGrowth arrest-specific 5 GAS5 was reported to be implicated and aberrantly express in multiple cancers. However, the expression and mechanism of action of GAS5 were largely poor understood in endometrial carcinoma.

ResultsAccording to the result of real-time reverse-transcriptase polymerase chain reaction RT-PCR and flow cytometry analysis, we identified that GAS5 was down-regulated in endometrial cancer cells and stimulated the apoptosis of endometrial cancer cells. To investigate the expression of GAS5, PTEN and miR-103, RT-PCR was performed. And we found that the expression of PTEN was up-regulated when endometrial cancer cells overexpressed GAS5. The prediction of bioinformatics online revealed that GAS5 could bind to miR-103, which was further found to be regulated by GAS5. Finally, we found that miR-103 mimic could decrease the mRNA and protein levels of PTEN through luciferase reporter assay and western blotting, and GAS5 plasmid may reverse this regulation effect in endometrial cancer cells.

ConclusionIn summary, we demonstrate that GAS5 acts as an tumor suppressor lncRNA in endometrial cancer. Through inhibiting the expression of miR-103, GAS5 significantly enhanced the expression of PTEN to promote cancer cell apoptosis, and, thus, could be an important mediator in the pathogenesis of endometrial cancer.

KeywordsLncRNA-GAS5 PTEN miR-103 Endometrial cancer Apoptosis AbbreviationsGAS5Growth arrest-specific 5

lncRNAsLong non-coding RNAs

NSCLCNon-small-cell lung cancer

PTENPhosphatase and tensin homologue

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Autor: Chen Guo - Wei-qi Song - Ping Sun - Lian Jin - Hong-yan Dai

Fuente: https://link.springer.com/







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