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BMC Cancer

, 7:180

First Online: 20 September 2007Received: 14 February 2007Accepted: 20 September 2007DOI: 10.1186-1471-2407-7-180

Cite this article as: Kumar, M.M., Ponvijay, K., Nandhini, R. et al. BMC Cancer 2007 7: 180. doi:10.1186-1471-2407-7-180

Abstract

BackgroundGeneration of novel spontaneous ER positive mammary tumor animal model from heterozygous NIH nude mice.

MethodsUsing brother-sister mating with pedigree expansion system, we derived a colony of heterozygous breeding females showing ER-Positive tumors around the age of 6 months. Complete blood picture, differential leukocyte count, and serum levels of Estrogen, Alanine amino transferase SGPT, Aspartate amino transferase SGOT, total protein and albumin were estimated. Aspiration biopsies and microbiology were carried out. Gross pathology of the tumors and their metastatic potential were assessed. The tumors were excised and further characterized using histopathology, cytology, electron microscopy EM, molecular markers and Mouse mammary Tumor Virus – Long Terminal Repeats MMTV LTR specific RT-PCR.

ResultsThe tumors originated from 2or 5or both the mammary glands and were multi-nodulated with variable central necrosis accompanied with an accumulation of inflammatory exudate. Significant increases in estrogen, SGPT, SGOT and neutrophils levels were noticed. Histopathologically, invasive nodular masses of pleomorphic tubular neoplastic epithelial cells invaded fibro-vascular stroma, adjacent dermis and subcutaneous tissue. Metastatic spread through hematogenous and regional lymph nodes, into liver, lungs, spleen, heart and dermal lymphatics was observed. EM picture revealed no viral particles and MMTV-negativity was confirmed through MMTV LTR-specific RT-PCR. High expression of ER α, moderate to high expression of proliferating cell nuclear antigen PCNA, moderate expression of vimentin and Cytokeratin 19 K19 and low expression of p53 were observed in tumor sections, when compared with that of the normal mammary gland.

ConclusionSince 75% of human breast cancer were classified ER-positive and as our model mimics in most of the characteristics, such as histopathology, metastasis, high estrogen levels the ER-positive luminal epithelial-like human breast cancer, this model will be an attractive tool to understand the biology of estrogen-dependant breast cancer in women. To our knowledge, this is the first report of a spontaneous mammary model displaying regional lymph node involvement with both hematogenous and lymphatic spread to liver, lung, heart, spleen and lymph nodes.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2407-7-180 contains supplementary material, which is available to authorized users.

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Autor: MJ Mahesh Kumar - KS Ponvijay - R Nandhini - RS Nagarajan - J Jose - G Srinivas - P Nagarajan - R Venkatesan - Kishor Ku

Fuente: https://link.springer.com/







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