Proto-oncogene HER-2 in normal, dysplastic and tumorous feline mammary glands: an immunohistochemical and chromogenic in situ hybridization studyReportar como inadecuado




Proto-oncogene HER-2 in normal, dysplastic and tumorous feline mammary glands: an immunohistochemical and chromogenic in situ hybridization study - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

BMC Cancer

, 7:179

First Online: 20 September 2007Received: 05 March 2007Accepted: 20 September 2007DOI: 10.1186-1471-2407-7-179

Cite this article as: Ordás, J., Millán, Y., Dios, R. et al. BMC Cancer 2007 7: 179. doi:10.1186-1471-2407-7-179

Abstract

BackgroundFeline mammary carcinoma has been proposed as a natural model of highly aggressive, hormone-independent human breast cancer. To further explore the utility of the model by adding new similarities between the two diseases, we have analyzed the oncogene HER-2 status at both the protein and the gene levels.

MethodsFormalin-fixed, paraffin-embedded tissue samples from 30 invasive carcinomas, 7 benign lesions and two normal mammary glands were analyzed. Tumour features with prognostic value were recorded. The expression of protein HER-2 was analyzed by immunohistochemistry and the number of gene copies by means of DNA chromogenic in situ hybridization.

ResultsImmunohistochemical HER-2 protein overexpression was found in 40% of feline mammary carcinomas, a percentage higher to that observed in human breast carcinoma. As in women, feline tumours with HER-2 protein overexpression had pathological features of high malignancy. However, amplification of HER-2 was detected in 16% of carcinomas with protein overexpression, a percentage much lower than that observed in their human counterpart.

ConclusionFeline mammary carcinoma would be a suitable natural model of that subset of human breast carcinomas with HER-2 protein overexpression without gene amplification.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2407-7-179 contains supplementary material, which is available to authorized users.

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Autor: Javier Ordás - Yolanda Millán - Rafaela Dios - Carlos Reymundo - Juana Martín de las Mulas

Fuente: https://link.springer.com/







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