In vitro antioxidant and free radical scavenging activity of different parts of Tabebuia pallida growing in BangladeshReport as inadecuate

In vitro antioxidant and free radical scavenging activity of different parts of Tabebuia pallida growing in Bangladesh - Download this document for free, or read online. Document in PDF available to download.

BMC Research Notes

, 8:621

Complementary and AlternativeMedicine


BackgroundIn humans, many diseases are associated with the accumulation of free radicals. Antioxidants can scavenge free radicals and minimize their impact. Therefore, the search for naturally occurring antioxidants of plant origin is imperative. Here, we aimed to investigate the antioxidant and free radical scavenging properties of methanolic extracts from Tabebuia pallida T. pallida stem bark TPSB, root bark TPRB, leaves TPL, and flowers TPF.

MethodsThe antioxidant and free radical scavenging activity were determined by several standard methods using spectrophotomer. Total phenolic and flavonoid contents were estimated using Folin-Ciocalteu reagent and aluminum chloride colorimetric assay methods, respectively.

ResultsAmong the extracts, TPL showed the highest total antioxidant capacity followed by TPRB, TPF, and TPSB. Based on DPPH and hydroxyl radical scavenging activity, TPL showed strong scavenging activity 91.05 ± 1.10 and 62.00 ± 0.57 with IC50 of 9.20 ± 0.28 and 46.00 ± 2.84 μg-mL, respectively when compared with standard BHT IC50 of 7.00 ± 0.25 μg-mL and CA 75.00 ± 0.14 μg-mL. These results suggest that TPL had the highest radical scavenging activity among the extractives that closely resembled the standard’s. In lipid peroxidation inhibition assay, TPL exhibited the most potent inhibitory activity 83.18 ± 2.12 % with IC50 of 12.00 ± 2.12 μg-mL, which closely resembled standard CA IC50 of 10.50 ± 0.28 μg-mL. Also, the reducing capacity on ferrous ion was in the following order: TPL > TPRB > TF > TPSB. The phenolic and flavonoid contents of TPL were higher than other extractives. A positive correlation pvalue <0.001 was observed between phenolic content and free radical DPPH and OH scavenging efficiencies and lipid peroxidation inhibition activity.

ConclusionMethanolic extract of T. pallida leaf is a potential source of natural antioxidants and serves as an effective free radical scavenger and-or inhibitor. Hence, T. pallida might be a good plant-based pharmaceutical product for several diseases caused by free radicals.

KeywordsTabebuia pallida Free radicals Polyphenols Antioxidant AbbreviationsAAascorbic acid


CAEcatechin equivalent


EDTAethylenediamine tetraacetic acid

BHTbutylated hydroxytoluene

FRACferrous reducing antioxidant capacity

GAgallic acid

GAEgallic acid equivalent

TACtotal antioxidant capacity

TCAtrichloro acetic acid

TBAthiobarbituric acid

TPLTabebuia pallida leaves

TPFTabebuia pallida flower

TPRBTabebuia pallida root bark

TSBTabebuia pallida stem bark

Electronic supplementary materialThe online version of this article doi:10.1186-s13104-015-1618-6 contains supplementary material, which is available to authorized users.

Download fulltext PDF

Author: Md. Mahbubur Rahman - Md. Badrul Islam - Mohitosh Biswas - A. H. M. Khurshid Alam


Related documents