Sequence Characterization of Mitochondrial 12S rRNA Gene in Mouse Deer Moschiola indica for PCR-RFLP Based Species IdentificationReportar como inadecuado




Sequence Characterization of Mitochondrial 12S rRNA Gene in Mouse Deer Moschiola indica for PCR-RFLP Based Species Identification - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

Molecular Biology InternationalVolume 2013 2013, Article ID 783925, 6 pages

Research Article

Centre for Wildlife, Indian Veterinary Research Institute, Izzatnagar, Uttar Pradesh 243 122, India

Department of Veterinary Physiology & Biochemistry, Veterinary College, KVAFSU, Hassan, Karnataka 573201, India

Division of Animal Biotechnology, Indian Veterinary Research Institute, Izzatnagar, Uttar Pradesh 243 122, India

Received 30 September 2013; Revised 20 November 2013; Accepted 20 November 2013

Academic Editor: Malayannan B. Subramaniam

Copyright © 2013 Chandra Mohan Siddappa et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Mitochondrial 12S rRNA has proven to be a useful molecular marker for better conservation and management of the endangered species. Polymerase chain reaction-restriction fragment length polymorphism PCR-RFLP of the mitochondrial 12S rRNA gene has proven to be a reliable and efficient tool for the identification of different Indian deer species of family cervidae. In the present study, mitochondrial 12S rRNA gene sequence of mouse deer Moschiola indica belonging to the family Tragulidae was characterized and analysed in silico for its use in species identification. Genomic DNA was isolated from the hair follicles and mitochondrial 12S rRNA gene was amplified using universal primers. PCR product was cloned and sequenced for the first time. The sequence of mouse deer showed 90.04, 90.08, 90.04, 91.2, 90.04, and 90.08% identities with sika deer, sambar, hog deer, musk deer, chital, and barking deer, respectively. Restriction mapping in Lasergene DNAstar Inc., Madison, WI, USA revealed that mouse deer mitochondrial 12S rRNA gene sequence can be differentiated from the other deer species in PCR-RFLP using RsaI, DdeI, BsrI, and BstSFI. With the help of predicted pattern, mouse deer can be identified using genomic DNA from a variety of biomaterials, thereby providing molecular aid in wildlife forensics and conservation of the species.





Autor: Chandra Mohan Siddappa, Mohini Saini, Asit Das, Ramesh Doreswamy, Anil K. Sharma, and Praveen K. Gupta

Fuente: https://www.hindawi.com/



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