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Journal of Translational Medicine

, 5:17

First Online: 28 March 2007Received: 09 January 2007Accepted: 28 March 2007DOI: 10.1186-1479-5876-5-17

Cite this article as: Slezak, S.L., Bettinotti, M., Selleri, S. et al. J Transl Med 2007 5: 17. doi:10.1186-1479-5876-5-17

Abstract

BackgroundAdoptive immune and vaccine therapies have been used to prevent cytomegalovirus CMV disease in recipients of hematopoietic progenitor cell transplants, but the nature of T cell responses to CMV have not been completely characterized.

MethodsPeptide pools and individual peptides derived from the immune-dominant CMV proteins pp65 and IE-1 and antigen-specific, cytokine flow cytometry were used to characterize the prevalence and frequency of CD4+ and CD8+ memory T cells in 20 healthy CMV-seropositive subjects.

ResultsCD8+ T cell responses to pp65 were detected in 35% of subjects and to IE-1 in 40% of subjects. CD4+ T cell responses to pp65 were detected in 50% of subjects, but none were detected to IE-1. Several new IE-1 HLA class I epitopes were identified, including 4 restricted to HLA-C antigens. One region of IE-1 spanning amino acids 300 to 327 was rich in class I epitopes. The HLA class I restrictions of IE-1 peptides were more promiscuous than those of pp65 peptides.

ConclusionSince naturally occurring CD4+ and CD8+ T cell responses to pp65 were detectable in many subjects, but only CD8+ T cell responses to IE-1 were detected, pp65 may be better than IE-1 for use in vaccine and adoptive immune therapies.

Electronic supplementary materialThe online version of this article doi:10.1186-1479-5876-5-17 contains supplementary material, which is available to authorized users.

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Autor: Stefanie L Slezak - Maria Bettinotti - Silvia Selleri - Sharon Adams - Francesco M Marincola - David F Stroncek

Fuente: https://link.springer.com/







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