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Journal of Nanomaterials - Volume 2016 2016, Article ID 7617894, 10 pages -

Research Article

Department of Radiological Science, Konyang University, Daejeon 302-718, Republic of Korea

College of Medicine, Konyang University, Daejeon 302-718, Republic of Korea

Received 30 December 2015; Revised 9 March 2016; Accepted 3 April 2016

Academic Editor: Muhammet S. Toprak

Copyright © 2016 Seong-Pyo Hong et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study aimed to synthesize theranostic agent targeting C6 rat glioma cell, which was based on the dextran coated paramagnetic gadolinium oxide nanoparticles D-PGONs conjugated with folic acid FA or paclitaxel PTX. The D-PGONs were synthesized by the in situ coprecipitation method, and the average value of the size distribution was 2.9 nm. FTIR spectroscopy was fulfilled to confirm the conjugations of FA or PTX with D-PGONs. The bioprotective effects of dextran coating and chemotherapeutic effect of PTX in the C6 glioma cell were evaluated by the MTT assay. The differences in uptakes between the synthesized theranostic agents into C6 cells were observed by confocal laser scanning microscopy. In addition, the magnetic contrast enhancement with different concentration of the synthesized agent was compared by the -weighted MRI imaging. It was experimentally shown that the synthesized theranostic agent targets C6 cells due to the ligand-receptor-mediated endocytosis and provides enhancement in MR contrast depending on the concentration due to the paramagnetic property of gadolinium nanoparticle. In addition, it was shown by the results of MTT assay that the synthesized nanocomposites were more effective in reducing cell viability than bare gadolinium nanoparticles. In conclusion, it was shown that FA and PTX conjugated D-PGONs could be used as the theranostic agent with paramagnetism and chemotherapeutic property.





Autor: Seong-Pyo Hong, Seong Hee Kang, Do Kyung Kim, and Bo Sun Kang

Fuente: https://www.hindawi.com/



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