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Journal of Translational Medicine

, 4:27

First Online: 04 July 2006Received: 30 March 2006Accepted: 04 July 2006DOI: 10.1186-1479-5876-4-27

Cite this article as: Gillis, J.S. J Transl Med 2006 4: 27. doi:10.1186-1479-5876-4-27

Abstract

BackgroundIn recent years encouraging progress has been made in developing vaccine treatments for cancer, particularly with melanoma. However, the overall rate of clinically significant results has remained low. The present research used microarray datasets from previous investigations to examine gene expression patterns in cancer cell lines with the goal of better understanding the tumor microenvironment.

MethodsPrincipal Components Analyses with Promax rotational transformations were carried out with 90 cancer cell lines from 3 microarray datasets, which had been made available on the internet as supplementary information from prior publications.

ResultsIn each of the analyses a well defined melanoma component was identified that contained a gene coding for the enzyme, glutaminyl cyclase, which was as highly expressed as genes from a variety of well established biomarkers for melanoma, such as MAGE-3 and MART-1, which have frequently been used in clinical trials of melanoma vaccines.

ConclusionSince glutaminyl cyclase converts glutamine and glutamic acid into a pyroglutamic form, it may interfere with the tumor destructive process of vaccines using peptides having glutamine or glutamic acid at their N-terminals. Finding ways of inhibiting the activity of glutaminyl cyclase in the tumor microenvironment may help to increase the effectiveness of some melanoma vaccines.

Electronic supplementary materialThe online version of this article doi:10.1186-1479-5876-4-27 contains supplementary material, which is available to authorized users.

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Autor: John Stuart Gillis

Fuente: https://link.springer.com/







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