A PCR assay detects a male-specific duplicated copy of Anti-Müllerian hormone amh in the lingcod Ophiodon elongatusReportar como inadecuado




A PCR assay detects a male-specific duplicated copy of Anti-Müllerian hormone amh in the lingcod Ophiodon elongatus - Descarga este documento en PDF. Documentación en PDF para descargar gratis. Disponible también para leer online.

BMC Research Notes

, 9:230

Genetics

Abstract

BackgroundAnti-Müllerian hormone amh or Müllerian-inhibiting substance mis is a member of the transforming growth factor-β family of hormones. This gene plays a key role in vertebrate male sex-determination by inhibiting the development of the Müllerian ducts, and has been shown to be the master sex-determinant in the Patagonian pejerrey.

ResultsIn the lingcod, Ophiodon elongatus, both males and females share one copy of amh, however we have identified a second duplicate copy that appears solely in the male individuals. We have developed a PCR-based assay targeting the TGF-β domain of amh that provides a simple method with which to sex lingcod from a small amount of tissue. An analysis across 57 individuals gave a 100 % success rate in identifying the phenotypic sex.

ConclusionsWe present a simple method to sex lingcod through non-lethal tissue sampling. A third, independent, male-specific duplication of amh in a teleost fish has been identified in the lingcod.

KeywordsSex determination amh Anti-Müllerian hormone Müllerian inhibiting substance mis Lingcod Ophiodon elongatus AbbreviationsAmhAnti-Müllerian hormone

MisMüllerian inhibiting substance

MSDmaster-sex determinant

TGF-βtransforming growth factor beta

UTRuntranslated region

Electronic supplementary materialThe online version of this article doi:10.1186-s13104-016-2030-6 contains supplementary material, which is available to authorized users.

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Autor: Eric B. Rondeau - Cassandra V. Laurie - Stewart C. Johnson - Ben F. Koop

Fuente: https://link.springer.com/



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