Knockdown of ST6Gal-I increases cisplatin sensitivity in cervical cancer cellsReportar como inadecuado




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BMC Cancer

, 16:949

Cell and molecular biology

Abstract

BackgroundSialyltransferase I ST6Gal-I is an enzyme involved in tumor metastasis that processes sialic acid precursors into their mature form, enabling them to regulate gene expression. However, the effect of ST6Gal-I on the biological behavior of cancer cells remain unclear. This study was the first to demonstrate the influence of ST6Gal-I on cisplatin sensitivity in cervical cancer cells.

MethodsKnockdown of ST6Gal-I was performed by shRNA and HeLa cells combination with cisplatin were tested.

ResultsWe showed that down-regulation of ST6Gal-I promoted cell apoptosis and inhibited proliferation and invasion in cervical cancer cells. Knockdown of ST6Gal-I by RNA interference increased the sensitivity of HeLa cells to cisplatin in vitro, and reduced tumor volume and suppressed subcutaneous tumor growth in response to cisplatin treatment in a xenograft mouse model in vivo.

ConclusionsThe results provide new information that ST6Gal-I plays an important role in several biological or pathological processes including drug resistance in cervical cancer and may be a potential therapeutic target to improve the response to chemotherapy in cervical cancer patients.

Keywordsα-2,6-sialic acid transferase DDP HeLa Apoptosis Invasion AbbreviationsANOVATwo-way analysis of variance

CMPCytidine monophosphate

ColCollagen

DDPCisplatin

DMEMDulbecco-s modified eagle-s medium

ECMExtracellular matrix

FITCFluorescein isothiocyanate

FnFibronectin

HEHematoxylin-eosin

IARCInternational agency for research on cancer

IC5050% inhibition rate

LnLaminin

MDRMultidrug resistance

MTT3-4,5-dimethylthiazol-2-yl-2-5-diphenyl tetrazolium bromide

NACNeoadjuvant chemotherapy

Neu5AcN-acetylneuraminic acids

PBSPhosphate-buffered saline

PIPropidium iodide

SDStandard deviation

shRNAShort hairpin RNA

SPSSStatistical package for the social sciences

ST6Gal-ISialyltransferase I

Electronic supplementary materialThe online version of this article doi:10.1186-s12885-016-2981-y contains supplementary material, which is available to authorized users.

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Autor: Xiaopeng Zhang - Chunchen Pan - Lei Zhou - Zhaogen Cai - Shufang Zhao - Donghong Yu

Fuente: https://link.springer.com/







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