Rheumatoid Factor Positivity Is Associated with Increased Joint Destruction and Upregulation of Matrix Metalloproteinase 9 and Cathepsin K Gene Expression in the Peripheral Blood in Rheumatoid Arthritic Patients Treated with MethoReport as inadecuate




Rheumatoid Factor Positivity Is Associated with Increased Joint Destruction and Upregulation of Matrix Metalloproteinase 9 and Cathepsin K Gene Expression in the Peripheral Blood in Rheumatoid Arthritic Patients Treated with Metho - Download this document for free, or read online. Document in PDF available to download.

International Journal of RheumatologyVolume 2013 2013, Article ID 457876, 10 pages

Clinical Study

Clinical Immunology Department, Nasonova Research Institute of Rheumatology, Russian Academy of Medical Sciences, Kashirskoye Shosse 34A, Moscow 115522, Russia

Early Rheumatoid Arthritis Department, Nasonova Research Institute of Rheumatology, Russian Academy of Medical Sciences, Moscow 115522, Russia

Vascular Pathology Department, Nasonova Research Institute of Rheumatology, Russian Academy of Medical Sciences, Moscow 115522, Russia

Received 31 March 2013; Revised 17 September 2013; Accepted 18 September 2013

Academic Editor: Ronald F. van Vollenhoven

Copyright © 2013 Elena V. Tchetina et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We evaluated changes in gene expression of mTOR, p21, caspase-3, ULK1, TNFα, matrix metalloproteinase MMP-9, and cathepsin K in the whole blood of rheumatoid arthritic RA patients treated with methotrexate MTX in relation to their rheumatoid factor status, clinical, immunological, and radiological parameters, and therapeutic response after a 24-month follow-up. The study group consisted of 35 control subjects and 33 RA patients without previous history of MTX treatment. Gene expression was measured using real-time RT-PCR. Decreased disease activity in patients at the end of the study was associated with significant downregulation of TNFα expression. Downregulation of mTOR was observed in seronegative patients, while no significant changes in the expression of p21, ULK1, or caspase-3 were noted in any RA patients at the end of the study. The increase in erosion numbers observed in the seropositive patients at the end of the follow-up was accompanied by upregulation of MMP-9 and cathepsin K, while seronegative patients demonstrated an absence of significant changes in MMP-9 and cathepsin K expression and no increase in the erosion score. Our results suggest that increased expression of MMP-9 and cathepsin K genes in the peripheral blood might indicate higher bone tissue destruction activity in RA patients treated with methotrexate. The clinical study registration number is 0120.0810610.





Author: Elena V. Tchetina, Natalia V. Demidova, Dmitry E. Karateev, and Eugeny L. Nasonov

Source: https://www.hindawi.com/



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