T-Cell and Antibody Responses to Mycobacterial Antigens in Tuberculin Skin-Test-Positive Bos indicus and Bos taurus Cattle in EthiopiaReport as inadecuate

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Veterinary Medicine InternationalVolume 2012 2012, Article ID 457872, 6 pages

Research Article

Aklilu Lemma Institute of Pathobiology, Addis Ababa University, P.O. Box 1176, Addis Ababa, Ethiopia

Pfizer Vaccine Research, PGRD, La Jolla, San Diego, CA 92121, USA

Chembio Diagnostic Systems, Inc., Research and Development Department, 3661 Horseblock Road, Medford, NY 11763, USA

Animal Health and Veterinary Laboratories Agency, Weybridge, New Haw, Addlestone, Surrey KT15 3NB, UK

Received 24 January 2012; Revised 4 March 2012; Accepted 20 March 2012

Academic Editor: Jesse M. Hostetter

Copyright © 2012 Gobena Ameni et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Higher IFN-γ responses to mycobacterial antigens were observed in Bos taurus Holsteins than in Bos indicus Zebu cattle which could due to differences in antigen recognition profiles between the two breeds. The present study was conducted to evaluate mycobacterial antigen recognition profiles of the two breeds. Twenty-three mycobacterial antigens were tested on 46 skin test positive 24 Zebu and 22 Holstein using enzyme-linked immunospot assay ELISPOT and multiple antigen print immunoassay MAPIA. Herds from which the study cattle obtained were tested for Fasciola antibody. The T cells from both breeds recognized most of the mycobacterial antigens at lower and comparable frequencies. However, antigens such as CFP-10, ESAT-6, Rv0287, Rv0288, MPB87, Acr-2, Rv3616c, and Rv3879c were recognized at higher frequencies in zebu while higher frequencies of T cell responses were observed to Hsp65 in both breeds. Furthermore, comparable antibody responses were observed in both breeds; MPB83 being the sero-dominant antigen in both breeds. The prevalence of Fasciola antibody was 81% and similar in both breeds. This piece of work could not lead to a definitive conclusion if there are differences in mycobacterial recognition profiles between the two breeds warranting for further similar studies using sound sample size from the two breeds.

Author: Gobena Ameni, Paul Cockle, Konstantin Lyashchenko, and Martin Vordermeier

Source: https://www.hindawi.com/


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