Expression of MicroRNAs in the Eyes of Lewis Rats with Experimental Autoimmune Anterior UveitisReport as inadecuate

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Mediators of InflammationVolume 2015 2015, Article ID 457835, 11 pages

Research Article

Department of Ophthalmology, Far Eastern Memorial Hospital, New Taipei City 22056, Taiwan

Department of Ophthalmology, National Taiwan University Hospital, Taipei 10002, Taiwan

National Taiwan University College of Medicine, Taipei 10002, Taiwan

Received 14 September 2015; Revised 12 November 2015; Accepted 16 November 2015

Academic Editor: Alex Kleinjan

Copyright © 2015 Yung-Ray Hsu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Purpose. This study aimed to determine the dynamic changes of NF-κB-related microRNAs miRNAs and cytokines over the course of experimental autoimmune anterior uveitis EAAU and elucidate the possible immunopathogenesis. Materials and Methods. Uveitis was induced in Lewis rats using bovine melanin-associated antigen. The inflammatory activity of the anterior chamber was clinically scored, and leukocytes in the aqueous humor were quantified. RNA was extracted from the iris-ciliary bodies and popliteal lymph nodes to reveal the dynamic changes of eight target miRNAs miR-155-5p, miR-146a-5p, miR-182-5p, miR-183-5p, miR-147b, miR-21-5p, miR-9-3p, and miR-223-3p and six cytokine mRNAs IFN-γ, IL-17, IL-12A, IL-1β, IL-6, and IL-10. In situ hybridization of miRNA and enzyme-linked immunosorbent assay quantification of cytokines were performed to confirm the results. Results. Disease activity and leukocyte quantification were maximum at day 15 after immunization. The profiling of miRNA revealed downregulation of miR-146a-5p, miR-155-5p, miR-223-3p, and miR-147b and upregulation of miR-182-5p, miR-183-5p, and miR-9-3p. Cytokine analysis revealed IFN-γ, IL-17, IL-12A, IL-1β, and IL-6 overexpression, with IL-10 downregulation. Conclusions. Dynamic changes of miRNAs were observed over the course of EAAU. By initiating NF-κB signaling, the expressions of downstream cytokines and effector cells from the Th17 and Th1 lineages were sequentially activated, contributing to the disease.

Author: Yung-Ray Hsu, Shu-Wen Chang, Yu-Cheng Lin, and Chang-Hao Yang



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