A novel method of modifying immune responses by vaccination with lipiodol-siRNA mixturesReport as inadecuate




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Journal of Translational Medicine

, 4:2

First Online: 03 January 2006Received: 21 October 2005Accepted: 03 January 2006DOI: 10.1186-1479-5876-4-2

Cite this article as: Ichim, T.E., Popov, I.A., Riordan, N.H. et al. J Transl Med 2006 4: 2. doi:10.1186-1479-5876-4-2

Abstract

The dendritic cell DC possesses the ability to stimulate both T helper 1 Th1 and Th2 responses depending on activation stimuli. Although it is known that chemically or genetically modified DC can be used therapeutically to steer immune responses towards either Th1 or Th2, cellular therapy with ex vivo manipulated DC is clinically difficult. Here we demonstrate a novel method of switching immune responses from Th1 to Th2 through in vivo immune modulation by administration of siRNA. We demonstrate that siRNA targeting of the IL-12p35 gene leads to a Th2 bias in vitro through an IL-10 dependent mechanism. In vivo administration of siRNA admixed with the oil-based contrast agent lipiodol in the presence of antigen and adjuvant induced a deviation in recall response to reduced production of IFN-γ and augmented IL-4 response using either KLH or ovalbumin. This simple method of in vivo modification of immune response possesses therapeutic potential in Th1-mediated diseases such as multiple sclerosis and autoimmune diabetes.

Electronic supplementary materialThe online version of this article doi:10.1186-1479-5876-4-2 contains supplementary material, which is available to authorized users.

Thomas E Ichim, Igor A Popov contributed equally to this work.

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Author: Thomas E Ichim - Igor A Popov - Neil H Riordan - Hamid Izadi - Zaohui Zhong - Li Yijian - Salman Sher - Eugenia K Olei

Source: https://link.springer.com/







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