Immature monocyte derived dendritic cells gene expression profile in response to Virus-Like Particles stimulationReport as inadecuate




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Journal of Translational Medicine

, 3:45

First Online: 29 December 2005Received: 30 September 2005Accepted: 29 December 2005DOI: 10.1186-1479-5876-3-45

Cite this article as: Aricò, E., Wang, E., Tornesello, M.L. et al. J Transl Med 2005 3: 45. doi:10.1186-1479-5876-3-45

Abstract

We have recently developed a candidate HIV-1 vaccine model based on HIV-1 Pr55gag Virus-Like Particles HIV-VLPs, produced in a baculovirus expression system and presenting a gp120 molecule from an Ugandan HIV-1 isolate of the clade A HIV-VLPAs.

The HIV-VLPAs induce in Balb-c mice systemic and mucosal neutralizing Antibodies as well as cytotoxic T lymphocytes, by intra-peritoneal as well as intra-nasal administration. Moreover, we have recently shown that the baculovirus-expressed HIV-VLPs induce maturation and activation of monocyte-derived dendritic cells MDDCs which, in turn, produce Th1- and Th2-specific cytokines and stimulate in vitro a primary and secondary response in autologous CD4+ T cells.

In the present manuscript, the effects of the baculovirus-expressed HIV-VLPAs on the genomic transcriptional profile of MDDCs obtained from normal healthy donors have been evaluated. The HIV-VLPA stimulation, compared to both PBS and LPS treatment, modulate the expression of genes involved in the morphological and functional changes characterizing the MDDCs activation and maturation.

The results of gene profiling analysis here presented are highly informative on the global pattern of gene expression alteration underlying the activation of MDDCs by HIV-VLPAs at the early stages of the immune response and may be extremely helpful for the identification of exclusive activation markers.

Electronic supplementary materialThe online version of this article doi:10.1186-1479-5876-3-45 contains supplementary material, which is available to authorized users.

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