The Effect of Parathion on Red Blood Cell Acetylcholinesterase in the Wistar RatReport as inadecuate

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Journal of Toxicology - Volume 2016 2016, Article ID 4576952, 5 pages -

Research Article

Sapporo Medical University, Sapporo 060-8556, Japan

Department of Emergency Medicine, University of Massachusetts Medical School, Worcester, MA 01655, USA

Received 12 February 2016; Revised 20 April 2016; Accepted 18 May 2016

Academic Editor: Orish Ebere Orisakwe

Copyright © 2016 Naofumi Bunya et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Organophosphorus OP pesticide poisoning is a significant problem worldwide. Research into new antidotes for these acetylcholinesterase inhibitors, and even optimal doses for current therapies, is hindered by a lack of standardized animal models. In this study, we sought to characterize the effects of the OP pesticide parathion on acetylcholinesterase in a Wistar rat model that included comprehensive medical care. Methods. Male Wistar rats were intubated and mechanically ventilated and then poisoned with between 20 mg-kg and 60 mg-kg of intravenous parathion. Upon developing signs of poisoning, the rats were treated with standard critical care, including atropine, pralidoxime chloride, and midazolam, for up to 48 hours. Acetylcholinesterase activity was determined serially for up to 8 days after poisoning. Results. At all doses of parathion, maximal depression of acetylcholinesterase occurred at 3 hours after poisoning. Acetylcholinesterase recovered to nearly 50% of baseline activity by day 4 in the 20 mg-kg cohort and by day 5 in the 40 and 60 mg-kg cohorts. At day 8, most rats’ acetylcholinesterase had recovered to roughly 70% of baseline. These data should be useful in developing rodent models of acute OP pesticide poisoning.

Author: Naofumi Bunya, Keigo Sawamoto, Hanif Benoit, and Steven B. Bird



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