The PBMC transcriptome profile after intake of oxidized versus high-quality fish oil: an explorative study in healthy subjectsReport as inadecuate




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Genes and Nutrition

, 11:16

First Online: 31 May 2016Received: 21 August 2015Accepted: 06 April 2016DOI: 10.1186-s12263-016-0530-6

Cite this article as: Myhrstad, M.C.W., Ottestad, I., Günther, CC. et al. Genes Nutr 2016 11: 16. doi:10.1186-s12263-016-0530-6

Abstract

BackgroundMarine long-chain polyunsaturated fatty acids are susceptible to oxidation, generating a range of different oxidation products with suggested negative health effects. The aim of the present study was to utilize sensitive high-throughput transcriptome analyses to investigate potential unfavorable effects of oxidized fish oil PV: 18 meq-kg; AV: 9 compared to high-quality fish oil PV: 4 meq-kg; AV: 3.

Methods In a double-blinded randomized controlled study for seven weeks, 35 healthy subjects were assigned to 8 g of either oxidized fish oil or high quality fish oil. The daily dose of EPA+DHA was 1.6 g. Peripheral blood mononuclear cells were isolated at baseline and after 7 weeks and transcriptome analyses were performed with the illuminaHT-12 v4 Expression BeadChip.

ResultsNo gene transcripts, biological processes, pathway or network were significantly changed in the oxidized fish oil group compared to the fish oil group. Furthermore, gene sets related to oxidative stress and cardiovascular disease were not differently regulated between the groups. Within group analyses revealed a more prominent effect after intake of high quality fish oil as 11 gene transcripts were significantly FDR < 0.1 changed from baseline versus three within the oxidized fish oil group.

ConclusionThe suggested concern linking lipid oxidation products to short-term unfavorable health effects may therefore not be evident at a molecular level in this explorative study.

Trial registrationClinicalTrials.gov, NCT01034423

KeywordsOxidized fish oil n-3 fatty acids PBMCs Transcriptome Human intervention AbbreviationsAVanisidine value

CHDcoronary heart disease

FCfold change

FDRfalse discovery rate

FOfish oil

LCPUFAslong-chain polyunsaturated fatty acids

meq-kgmilliequivalents per kilogram

oxFOoxidized fish oil

PBMCsperipheral blood mononuclear cells

PVperoxide value

Electronic supplementary materialThe online version of this article doi:10.1186-s12263-016-0530-6 contains supplementary material, which is available to authorized users.

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Author: Mari C. W. Myhrstad - Inger Ottestad - Clara-Cecilie Günther - Einar Ryeng - Marit Holden - Astrid Nilsson - Kirsti W. 

Source: https://link.springer.com/







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