Expression of minichromosome maintenance protein 2 as a marker for proliferation and prognosis in diffuse large B-cell lymphoma: a tissue microarray and clinico-pathological analysisReport as inadecuate




Expression of minichromosome maintenance protein 2 as a marker for proliferation and prognosis in diffuse large B-cell lymphoma: a tissue microarray and clinico-pathological analysis - Download this document for free, or read online. Document in PDF available to download.

BMC Cancer

, 5:162

First Online: 20 December 2005Received: 04 August 2005Accepted: 20 December 2005DOI: 10.1186-1471-2407-5-162

Cite this article as: Obermann, E.C., Went, P., Zimpfer, A. et al. BMC Cancer 2005 5: 162. doi:10.1186-1471-2407-5-162

Abstract

BackgroundMinichromosome maintenance MCM proteins are essential for the initiation of DNA replication and have been found to be relevant markers for prognosis in a variety of tumours. The aim of this study was to assess the proliferative activity of diffuse large B-cell lymphoma DLBCL in tissue microarray TMA using one of the minichromosome maintenance proteins Mcm2 and to explore its potential value to predict prognosis.

MethodsImmunohistochemistry for Mcm2 was performed on TMAs constructed from 302 cases of DLBCL. A monoclonal mouse antibody was used after heat induced antigen retrieval. Mcm2 expression was scored quantitatively. Positivity for Mcm2 was defined as presence of nuclear expression of Mcm2 in greater than or equal to 40 % of tumour cells. A statistical analysis was carried out of the association of Mcm2 and the clinico-pathological characteristics.

ResultsMcm2 expression was clearly evident in the nuclei of proliferating non-neoplastic cells and tumour cells. Positivity for Mcm2 was found in 46% 98-211 of analysable cases. A significant correlation existed between Mcm2 expression and presence of bulky disease p = 0.003. Poor disease specific survival was observed in patients with DLBCL positive for Mcm2 expression in the univariate analysis p = 0.0424.

ConclusionMcm2 expression can be used to assess tumour proliferation and may be useful as an additional prognostic marker to refine the prediction of outcome in DLBCL.

Electronic supplementary materialThe online version of this article doi:10.1186-1471-2407-5-162 contains supplementary material, which is available to authorized users.

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Author: Ellen C Obermann - Philip Went - Annette Zimpfer - Alexandar Tzankov - Peter J Wild - Robert Stoehr - Stefano A Pileri -

Source: https://link.springer.com/







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