Post-mortem computed tomography coaxial cutting needle biopsy to facilitate the detection of bacterioplankton using PCR probes as a diagnostic indicator for drowningReport as inadecuate




Post-mortem computed tomography coaxial cutting needle biopsy to facilitate the detection of bacterioplankton using PCR probes as a diagnostic indicator for drowning - Download this document for free, or read online. Document in PDF available to download.

International Journal of Legal Medicine

, Volume 131, Issue 1, pp 211–216

First Online: 05 November 2016Received: 07 March 2016Accepted: 11 October 2016DOI: 10.1007-s00414-016-1473-6

Cite this article as: Rutty, G.N., Johnson, C., Amoroso, J. et al. Int J Legal Med 2017 131: 211. doi:10.1007-s00414-016-1473-6

Abstract

We report for the first time the use of coaxial cutting needle biopsy, guided by post-mortem computed tomography PMCT, to sample internal body tissues for bacterioplankton PCR analysis to investigate drowning. This technical report describes the biopsy technique, the comparison of the needle biopsy and the invasive autopsy sampling results, as well as the PMCT and autopsy findings. By using this new biopsy sampling approach for bacterioplankton PCR, we have developed on previous papers describing the minimally invasive PMCT approach for the diagnosis of drowning. When such a system is used, the operator must take all precautions to avoid contamination of the core biopsy samples due to the sensitivity of PCR-based analytic systems.

KeywordsForensic Post-mortem computed tomography PMCT Coaxial cutting needle biopsy Drowning Bacterioplankton Real-time PCR Guy N. Rutty and Bruno Morgan contributed equally to this work.

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Author: Guy N. Rutty - Christopher Johnson - Jasmin Amoroso - Claire Robinson - Carina J. Bradley - Bruno Morgan

Source: https://link.springer.com/







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